Phospho-Aconitase 1 (Ser138) Antibody
(Synonyms: IREB1, ACO1, Cytoplasmic aconitate hydratase, Aconitase, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, Iron-responsive element-binding protein 1, IRP1, IRE-BP 1)Phospho-Aconitase 1 (Ser138) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Phospho-Aconitase 1 (Ser138).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:100-500 |
Product Details
Phospho-Aconitase 1 (Ser138) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Phospho-Aconitase 1 (Ser138).
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 100 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 98 kDa
Synthetic phosphopeptide corresponding to residues surrounding S138 of human Aconitase 1 protein.
Endogenous
affinity purified.
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Aconitase 1 is a Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA[1][2][3][4].
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Subcellular Localization
Cytoplasm, cytosol
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Isoforms & Post-Translational Modification
Phospho-Aconitase 1 has an amino acid length of 889, molecular weight is 98399 Da.
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Subunit
Interacts (when associated with the 4Fe-4S) with FBXL5.
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SwissProt ID
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Synonyms
IREB1, ACO1, Cytoplasmic aconitate hydratase, Aconitase, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, Iron-responsive element-binding protein 1, IRP1, IRE-BP 1
Documentation
References
[1]. Haile DJ, et al. Cellular regulation of the iron-responsive element binding protein: disassembly of the cubane iron-sulfur cluster results in high-affinity RNA binding. Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11735-9. [Content Brief]
[2]. Kaptain S, et al. A regulated RNA binding protein also possesses aconitase activity. Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10109-13. [Content Brief]
[3]. Philpott CC, et al. The bifunctional iron-responsive element binding protein/cytosolic aconitase: the role of active-site residues in ligand binding and regulation. Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7321-5. [Content Brief]
[4]. Stehling O, et al. Human CIA2A-FAM96A and CIA2B-FAM96B integrate iron homeostasis and maturation of different subsets of cytosolic-nuclear iron-sulfur proteins. Cell Metab. 2013 Aug 6;18(2):187-98. [Content Brief]
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