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FGFR2 Antibody (YA764)

(Synonyms: CD332, BEK, KGFR, KSAM, FGFR2, Fibroblast growth factor receptor 2, FGFR-2, K-sam, Keratinocyte growth factor receptor)
Customer Review

Based on 1 Customer Validation

FGFR2 Antibody (YA764) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FGFR2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for FGFR2 Antibody (YA764)
  • Experimental Validation Results for FGFR2 Antibody (YA764)
  • Experimental Validation Results for FGFR2 Antibody (YA764)
  • FGFR2 Antibody (YA764) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500 1:100-1:200 1:100-1:200

Product Details

Description

FGFR2 Antibody (YA764) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FGFR2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 140 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 92 kDa；
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human FGFR2 / CD332.AA range:601-723.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102483

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FGFR2 Antibody (YA764)
    Western blot analysis of extracts from HeLa(lane2(20μg) , Jurkat(lane 3(20μg) and K-562(lane 4(20ug) using FGFR2/CD332 Antibody (HY-P80398) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FGFR2 Antibody (YA764)
    Immunocytochemistry analysis of SK-BR-3 cells labeling CD332 with CD332 Antibody (HY-P80398)at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD332 Antibody (HY-P80398) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FGFR2 Antibody (YA764)
    Immunocytochemistry analysis of SK-BR-3 cells labeling CD332 with CD332 Antibody (HY-P80398) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD332 Antibody (HY-P80398) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    FGFR2 encodes FGFR2b and FGFR2c isoforms with distinct expression domains and ligand specificity[1]. Mechanistically, FGF/FGFR signaling regulates lineage commitment, differentiation, proliferation, and apoptosis in development and adult homeostasis[2]. In disease models, FGFR2 alterations activate signaling in breast, gastric, cholangiocarcinoma, lung squamous, and oral squamous cancers[1][3][4]. Compared with related isoforms, FGFR2b-to-FGFR2c switching occurs during prostate and bladder cancer progression, linking splice regulation to epithelial-mesenchymal transition[1]. For experimental applications, FGFR2 mutation or amplification supports use of FGFR inhibitors, while extracellular-domain FGFR2 mutations can drive constitutive dimerization and inhibitor-sensitive transformation[1][4].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Golgi apparatus; Cytoplasmic vesicle; Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Secreted; Secreted

  • Subunit

    Monomer. Homodimer after ligand binding. Interacts predominantly with FGF1 and FGF2, but can also interact with FGF3, FGF4, FGF6, FGF7, FGF8, FGF9, FGF10, FGF17, FGF18 and FGF22 (in vitro). Ligand specificity is determined by tissue-specific expression of isoforms, and differences in the third Ig-like domain are crucial for ligand specificity. Isoform 1 has high affinity for FGF1 and FGF2, but low affinity for FGF7. Isoform 3 has high affinity for FGF1 and FGF7, and has much higher affinity for FGF7 than isoform 1 (in vitro). Affinity for fibroblast growth factors (FGFs) is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Likewise, KLB increases the affinity for FGF19 and FGF21. Interacts with PLCG1, GRB2 and PAK4. Interacts with FLRT2 (By similarity)

  • SwissProt ID

    P21802

  • Gene ID
    2263 [NCBI]
  • Synonyms

    CD332, BEK, KGFR, KSAM, FGFR2, Fibroblast growth factor receptor 2, FGFR-2, K-sam, Keratinocyte growth factor receptor

  • Research Field

    Cardiovascular

[1]. Katoh Y, et al. FGFR2-related pathogenesis and FGFR2-targeted therapeutics (Review). Int J Mol Med. 2009 Mar;23(3):307-11. [Content Brief]

[2]. Xie Y, et al. FGF/FGFR signaling in health and disease. Signal Transduct Target Ther. 2020 Sep 2;5(1):181. [Content Brief]

[3]. Egan JB, et al. Molecular Modeling and Functional Analysis of Exome Sequencing-Derived Variants of Unknown Significance Identify a Novel, Constitutively Active FGFR2 Mutant in Cholangiocarcinoma. JCO Precis Oncol. 2017;2017. [Content Brief]

[4]. Liao RG, et al. Inhibitor-sensitive FGFR2 and FGFR3 mutations in lung squamous cell carcinoma. Cancer Res. 2013 Aug 15;73(16):5195-205. [Content Brief]

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FGFR2 Antibody (YA764) Related Classifications

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Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane2(20μg) , Jurkat(lane 3(20μg) and K-562(lane 4(20ug) using FGFR2/CD332 Antibody (HY-P80398) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of SK-BR-3 cells labeling CD332 with CD332 Antibody (HY-P80398)at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD332 Antibody (HY-P80398) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of SK-BR-3 cells labeling CD332 with CD332 Antibody (HY-P80398) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD332 Antibody (HY-P80398) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FGFR2 Antibody (YA764) Product Image
    1/16
  • Experimental Validation Results for FGFR2 Antibody (YA764)
  • Experimental Validation Results for FGFR2 Antibody (YA764)
  • Experimental Validation Results for FGFR2 Antibody (YA764)
  • FGFR2 Antibody (YA764) Product Image
  • Experimental Validation Results for FGFR2 Antibody (YA764)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane2(20μg) , Jurkat(lane 3(20μg) and K-562(lane 4(20ug) using FGFR2/CD332 Antibody (HY-P80398) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FGFR2 Antibody (YA764)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of SK-BR-3 cells labeling CD332 with CD332 Antibody (HY-P80398)at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD332 Antibody (HY-P80398) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FGFR2 Antibody (YA764)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of SK-BR-3 cells labeling CD332 with CD332 Antibody (HY-P80398) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD332 Antibody (HY-P80398) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FGFR2 Antibody (YA764)
  • Experimental Validation Results for FGFR2 Antibody (YA764)
  • Experimental Validation Results for FGFR2 Antibody (YA764)
  • FGFR2 Antibody (YA764) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa(lane2(20μg) , Jurkat(lane 3(20μg) and K-562(lane 4(20ug) using FGFR2/CD332 Antibody (HY-P80398) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • FGFR2 Antibody (YA764) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of SK-BR-3 cells labeling CD332 with CD332 Antibody (HY-P80398)at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD332 Antibody (HY-P80398) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FGFR2 Antibody (YA764) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of SK-BR-3 cells labeling CD332 with CD332 Antibody (HY-P80398) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD332 Antibody (HY-P80398) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FGFR2 Antibody (YA764) Product Image WB
  • FGFR2 Antibody (YA764) Product Image ICC
  • FGFR2 Antibody (YA764) Product Image ICC