Phospho-Chk2 (Thr68) Antibody (YA7410)

Cat. No.: HY-P87725: Ficha de datos User Guide for Antibodies
FAQs
Technical Support

Phospho-Chk2 (Thr68) Antibody (YA7410) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-Chk2 (Thr68).

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

* Seleccione Cantidad antes de agregar artículos.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

Phospho-Chk2 (Thr68) Antibody (YA7410) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-Chk2 (Thr68).

Host

Rabbit

Clonality

Monoclonal,Recombinant

Peso molecular

Predicted band size: 61 kDa;

Observed band size: 61 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O96017

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200

Sensitivity	Endogenous	Pureza	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG, Kappa

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T] (PubMed:37943659). Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed:25361978). Under oxidative stress, promotes ATG7 ubiquitination by phosphorylating the E3 ubiquitin ligase TRIM32 at 'Ser-55' leading to positive regulation of the autophagosme assembly (PubMed:37943659)|(Microbial infection) Phosphorylates herpes simplex virus 1/HHV-1 protein ICP0 and thus activates its SUMO-targeted ubiquitin ligase activity

Subcellular Localization：Nucleus,Nucleus,Nucleus,Nucleus,Nucleus,Nucleus, PML body,Nucleus, nucleoplasm

Expression：
Tissue_Specificity: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues

Isoforms & Post-Translational Modification：O96017 has 13 isomers: O96017-1: 60915 Da (predicted); O96017-2: 26084 Da (predicted); O96017-3: 17370 Da (predicted); O96017-4: 50203 Da (predicted); O96017-5: 22594 Da (predicted); O96017-6: 18706 Da (predicted); O96017-7: 38125 Da (predicted); O96017-8: 32142 Da (predicted); O96017-9: 65419 Da (predicted); O96017-10: 15420 Da (predicted); O96017-11: 24396 Da (predicted). O96017-12: 57526 Da (predicted); O96017-13: 36157 Da (predicted).
Phosphorylated丨Ubiquitinated

Subunit：Homodimer

Documentación

User Guide for Antibodies (1077 KB)