IGFBP1 Antibody (YA2410)

Cat. No.: HY-P82665: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

IGFBP1 Antibody (YA2410) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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IGFBP1 Antibody (YA2410) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

IGFBP1 Antibody (YA2410) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 28 kDa;

Observed band size: 28 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P08833

Gene ID

3484 [NCBI]

Immunogen

A synthesized peptide derived from human IGFBP1 aa215-259.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using IGFBP1 Antibody (HY-P82665) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including cell migration, proliferation, differentiation or apoptosis in a cell-type specific manner (PubMed:11397844, PubMed:15972819). Also plays a positive role in cell migration by interacting with integrin ITGA5:ITGB1 through its RGD motif (PubMed:7504269). Mechanistically, binding to integrins leads to activation of focal adhesion kinase/PTK2 and stimulation of the mitogen-activated protein kinase (MAPK) pathway (PubMed:11397844). Regulates cardiomyocyte apoptosis by suppressing HIF-1alpha/HIF1A ubiquitination and subsequent degradation (By similarity)

Subcellular Localization：Secreted

Subunit：Binds equally well IGF1 and IGF2 (PubMed:7679979). Interacts with integrin ITGA5:ITGB1 (PubMed:7504269). Interacts with VHL; this interaction inhibits HIF1A degradation (By similarity)

RRID

AB_3104765

Database

Entrez Gene: 3484 Human ; 16006 Mouse ; 25685 Rat

SwissProt: P08833 Human ; P47876 Mouse ; P21743 Rat

OMIM: 146730 Human

Research Field

Signal Transduction

Synonyms

IGFBP1; IBP1; Insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; IGFBP-1; Placental protein 12; PP12

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

IGFBP1 Antibody (YA2410) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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IGFBP1 Antibody (YA2410)

IGFBP1 Antibody (YA2410) Featured Recommendations:

Product Detail

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Background

Documentation

IGFBP1 Antibody (YA2410) Related Classifications

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