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EGR2 Antibody (YA1926)

(Synonyms: EGR2; KROX20; E3 SUMO-protein ligase EGR2; AT591; E3 SUMO-protein transferase ERG2 Curated; Early growth response protein 2 (EGR-2); Zinc finger protein Krox-20; )
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Based on 1 Customer Validation

EGR2 Antibody (YA1926) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EGR2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for EGR2 Antibody (YA1926)
  • EGR2 Antibody (YA1926) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

EGR2 Antibody (YA1926) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EGR2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 53 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human EGR2 aa2-56.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104294

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EGR2 Antibody (YA1926)
    Western blot analysis was performed on extracts from U251MG (lane 1, 15 μg), LnCaP (lane 2, 15 μg), K562 (lane 3, 15 μg), HepG2 (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using EGR2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    EGR2 is a Sequence-specific DNA-binding transcription factor. Plays a role in hindbrain segmentation by regulating the expression of a subset of homeobox containing genes and in Schwann cell myelination by regulating the expression of genes involved in the formation and maintenance of myelin. Binds to two EGR2-consensus sites EGR2A (5'-CTGTAGGAG-3') and EGR2B (5'-ATGTAGGTG-3') in the HOXB3 enhancer and promotes HOXB3 transcriptional activation. Binds to specific DNA sites located in the promoter region of HOXA4, HOXB2 and ERBB2. Regulates hindbrain segmentation by controlling the expression of Hox genes, such as HOXA4, HOXB3 and HOXB2, and thereby specifying odd and even rhombomeres. Promotes the expression of HOXB3 in the rhombomere r5 in the hindbrain. Regulates myelination in the peripheral nervous system after birth, possibly by regulating the expression of myelin proteins, such as MPZ, and by promoting the differentiation of Schwann cells. Involved in the development of the jaw openener musculature, probably by playing a role in its innervation through trigeminal motor neurons. May play a role in adipogenesis, possibly by regulating the expression of CEBPB; E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 transcriptional activity[1].

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    P11161 has 2 isomers: P11161-1: 50302 Da (predicted); P11161-2: 44970 Da (predicted).
    Ubiquitinated by WWP2 leading to proteasomal degradation;Acetylated at Lys-247. May be deacetylated by HDAC6, HDAC10 or SIRT1

  • Subunit

    Interacts with HCFC1 (PubMed:14532282). Interacts with WWP2 (PubMed:19651900). Interacts with UBC9 (PubMed:21836637). Interacts with CITED1 (By similarity). Interacts (via phosphorylated form) with SFN (By similarity)

  • SwissProt ID

    P11161

  • Gene ID
    1959 [NCBI]
  • Synonyms

    EGR2; KROX20; E3 SUMO-protein ligase EGR2; AT591; E3 SUMO-protein transferase ERG2 Curated; Early growth response protein 2 (EGR-2); Zinc finger protein Krox-20;

  • Research Field

    Epigenetics and Nuclear Signaling

EGR2 Antibody (YA1926) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from U251MG (lane 1, 15 μg), LnCaP (lane 2, 15 μg), K562 (lane 3, 15 μg), HepG2 (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using EGR2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.

  • EGR2 Antibody (YA1926) Product Image
    1/16
  • Experimental Validation Results for EGR2 Antibody (YA1926)
  • EGR2 Antibody (YA1926) Product Image
  • Experimental Validation Results for EGR2 Antibody (YA1926)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from U251MG (lane 1, 15 μg), LnCaP (lane 2, 15 μg), K562 (lane 3, 15 μg), HepG2 (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using EGR2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for EGR2 Antibody (YA1926)
  • EGR2 Antibody (YA1926) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from U251MG (lane 1, 15 μg), LnCaP (lane 2, 15 μg), K562 (lane 3, 15 μg), HepG2 (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using EGR2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.

  • EGR2 Antibody (YA1926) Product Image WB