Protein C Antibody (YA2461)

Cat. No.: HY-P82716: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Protein C Antibody (YA2461) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Protein C.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Protein C Antibody (YA2461) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Protein C Antibody (YA2461) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Protein C.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 52 kDa;

Observed band size: 52 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P04070

Gene ID

5624 [NCBI]

Immunogen

A synthesized peptide derived from human Protein C aa300-360.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using Protein C Antibody (HY-P82716) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845)

Subcellular Localization：Secreted; Golgi apparatus; Endoplasmic reticulum

Expression：
Tissue_specificity:Plasma; synthesized in the liver

Isoforms & Post-Translational Modification：P04070 has 2 isomers: P04070-1: 52071 Da (predicted); P04070-2: 57556 Da (predicted).
The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium;N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans;The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains;May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide

Subunit：Synthesized as a single chain precursor, which is cleaved into a light chain and a heavy chain held together by a disulfide bond. The enzyme is then activated by thrombin, which cleaves a tetradecapeptide from the amino end of the heavy chain; this reaction, which occurs at the surface of endothelial cells, is strongly promoted by thrombomodulin. Interacts (activated) with iripin-8, a serine protease inhibitor from Ixodes ricinus saliva (PubMed:34502392)

RRID

AB_3104815

Database

Entrez Gene: 5624 Human

SwissProt: P04070 Human

OMIM: 176860 Human

Research Field

Cardiovascular

Synonyms

PROC; Vitamin K-dependent protein C; Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV

Documentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Protein C Antibody (YA2461) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Protein C Antibody (YA2461)

Protein C Antibody (YA2461) Featured Recommendations:

Product Detail

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Protein C Antibody (YA2461) Related Classifications

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