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IDH1 Antibody (YA3516)

(Synonyms: PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase)
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Based on 1 publication(s) in Google Scholar

IDH1 Antibody (YA3516) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IDH1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for IDH1 Antibody (YA3516)
  • Experimental Validation Results for IDH1 Antibody (YA3516)
  • Experimental Validation Results for IDH1 Antibody (YA3516)
  • IDH1 Antibody (YA3516) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

IDH1 Antibody (YA3516) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IDH1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human IDH1 aa1-70/414.

Sensitivity

Endogenous

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102017

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IDH1 Antibody (YA3516)
    Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using IDH1 Antibody (HY-P80995) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IDH1 Antibody (YA3516)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using IDH1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IDH1 Antibody (YA3516)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using IDH1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    IDH1 (isocitrate dehydrogenase 1) is a cytosolic and peroxisomal NADP+-dependent enzyme that catalyzes the conversion of isocitrate to α-ketoglutarate (α-KG) while generating NADPH, thereby supporting cellular redox homeostasis and intermediary metabolism[1][2]. Mechanistically, IDH1 contributes to metabolic pathways that regulate oxidative stress responses, biosynthetic processes, and α-KG availability for multiple cellular reactions[1][2]. In cancer biology, IDH1 is notable because recurrent mutations, most commonly affecting residue R132, confer a neomorphic enzymatic activity that converts α-KG into D-2-hydroxyglutarate (D-2HG), an oncometabolite that accumulates in tumor cells[3][2][4]. Elevated D-2HG inhibits α-KG-dependent dioxygenases, resulting in widespread epigenetic alterations and impaired cellular differentiation that contribute to tumor development and progression[2][4][5]. These molecular effects have been extensively studied in glioma and acute myeloid leukemia models, where mutant IDH1 functions as an early driver of tumorigenesis and metabolic reprogramming[4][5][6]. Compared with the related isoform IDH2, which is localized primarily in mitochondria, IDH1 is predominantly cytosolic and peroxisomal, creating distinct subcellular metabolic functions despite sharing similar catalytic chemistry[1][7]. For experimental applications, selective mutant IDH1 inhibitors reduce D-2HG production and promote cellular differentiation in preclinical models, making them valuable tools for investigating IDH1-dependent metabolic and epigenetic mechanisms in cancer research[5][6][8].

  • Subcellular Localization

    Cytoplasm, cytosol; Peroxisome

  • Subunit

    Homodimer

  • SwissProt ID

    O75874

  • Gene ID
    3417 [NCBI]
  • Synonyms

    PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

[1]. IDH1 gene information from NCBI.

[2]. Alzial G, et al. Wild-type isocitrate dehydrogenase under the spotlight in glioblastoma. Oncogene. 2022 Jan;41(5):613-621. [Content Brief]

[3]. Liu Z, et al. Inhibition of cancer-associated mutant isocitrate dehydrogenases: synthesis, structure-activity relationship, and selective antitumor activity. J Med Chem. 2014 Oct 23;57(20):8307-18. [Content Brief]

[4]. Waitkus MS, et al. Biological Role and Therapeutic Potential of IDH Mutations in Cancer. Cancer Cell. 2018 Aug 13;34(2):186-195. [Content Brief]

[5]. Rohle D, et al. An inhibitor of mutant IDH1 delays growth and promotes differentiation of glioma cells. Science. 2013 May 3;340(6132):626-30. [Content Brief]

[6]. Golub D, et al. Mutant Isocitrate Dehydrogenase Inhibitors as Targeted Cancer Therapeutics. Front Oncol. 2019 May 17;9:417. [Content Brief]

[7]. Al-Khallaf H. Isocitrate dehydrogenases in physiology and cancer: biochemical and molecular insight. Cell Biosci. 2017;7:37.

[8]. Popovici-Muller J, et al. Discovery of the First Potent Inhibitors of Mutant IDH1 That Lower Tumor 2-HG in Vivo. ACS Med Chem Lett. 2012 Sep 17;3(10):850-5. [Content Brief]

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IDH1 Antibody (YA3516) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using IDH1 Antibody (HY-P80995) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using IDH1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using IDH1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IDH1 Antibody (YA3516) Product Image
    1/16
  • Experimental Validation Results for IDH1 Antibody (YA3516)
  • Experimental Validation Results for IDH1 Antibody (YA3516)
  • Experimental Validation Results for IDH1 Antibody (YA3516)
  • IDH1 Antibody (YA3516) Product Image
  • Experimental Validation Results for IDH1 Antibody (YA3516)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using IDH1 Antibody (HY-P80995) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IDH1 Antibody (YA3516)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using IDH1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IDH1 Antibody (YA3516)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using IDH1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IDH1 Antibody (YA3516)
  • Experimental Validation Results for IDH1 Antibody (YA3516)
  • Experimental Validation Results for IDH1 Antibody (YA3516)
  • IDH1 Antibody (YA3516) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using IDH1 Antibody (HY-P80995) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • IDH1 Antibody (YA3516) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using IDH1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IDH1 Antibody (YA3516) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using IDH1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IDH1 Antibody (YA3516) Product Image WB
  • IDH1 Antibody (YA3516) Product Image IHC-P
  • IDH1 Antibody (YA3516) Product Image IHC-P