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AIM2 Antibody (YA3513)(PBS only)

(Synonyms: PYHIN4)

AIM2 Antibody (YA3513) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AIM2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
  • AIM2 Antibody (YA3513)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:10000

Product Details

Description

AIM2 Antibody (YA3513) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AIM2.

  • Host Mouse
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 39 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39 kDa
Immunogen

Purified recombinant fragment of human AIM2 (AA: 1-195) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
    Western blot analysis of extracts from A431 (lane 2(20μg), Hela (lane 3(20μg), A549 (lane 4(20μg) and HepG2 (lane 5(20μg) using AIM2 Antibody (HY-P83816) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
    Immunocytochemistry analysis of A549 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
    Immunocytochemistry analysis of MCF-7 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    AIM2 is a cytosolic innate immune receptor which recognizes double-stranded DNA (dsDNA) released during cellular perturbation and pathogenic assault.

  • Subcellular Localization

    Cytoplasm; Inflammasome; Nucleus

  • Expression


    Tissue_specificity:Expressed in spleen, small intestine, peripheral blood leukocytes, and testis

    Induction:By IFNG/IFN-gamma and IFNB1/IFN-beta

  • Subunit

    Self-associates; forms homooligomers in response to cytosolic double-stranded DNA (dsDNA) and the dsDNA seems to serve as oligomerization platform (PubMed:15582594, PubMed:19158676, PubMed:24406744, PubMed:26197926, PubMed:26583071, PubMed:29440442, PubMed:33980849).

  • SwissProt ID

    O14862

  • Gene ID
    9447 [NCBI]
  • Synonyms

    PYHIN4

AIM2 Antibody (YA3513)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 2(20μg), Hela (lane 3(20μg), A549 (lane 4(20μg) and HepG2 (lane 5(20μg) using AIM2 Antibody (HY-P83816) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • AIM2 Antibody (YA3513)(PBS only) Product Image
    1/16
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
  • AIM2 Antibody (YA3513)(PBS only) Product Image
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 2(20μg), Hela (lane 3(20μg), A549 (lane 4(20μg) and HepG2 (lane 5(20μg) using AIM2 Antibody (HY-P83816) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
  • Experimental Validation Results for AIM2 Antibody (YA3513)(PBS only)
  • AIM2 Antibody (YA3513)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431 (lane 2(20μg), Hela (lane 3(20μg), A549 (lane 4(20μg) and HepG2 (lane 5(20μg) using AIM2 Antibody (HY-P83816) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • AIM2 Antibody (YA3513)(PBS only) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • AIM2 Antibody (YA3513)(PBS only) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling AIM2 with AIM2 Antibody (HY-P83816) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with AIM2 Antibody (HY-P83816) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • AIM2 Antibody (YA3513)(PBS only) Product Image WB
  • AIM2 Antibody (YA3513)(PBS only) Product Image ICC
  • AIM2 Antibody (YA3513)(PBS only) Product Image ICC