Yes1 Antibody (YA4808)

Cat. No.: HY-P85116: Data Sheet User Guide for Antibodies
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Yes1 Antibody (YA4808) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Yes1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Yes1 Antibody (YA4808) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Yes1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 61 kDa;

Species Reactivity

Human

SwissProt ID

P07947

Gene ID

7525 [NCBI]

Immunogen

Purified recombinant fragment of c-Yes (aa10-193) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGFR, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. Catalyzes phosphorylation of organic cation transporter OCT2 which induces its transport activity (PubMed:26979622)

Subcellular Localization：Cell membrane; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytosol; Cell junction

Expression：
Tissue_specificity:In fetal tissues, this gene is expressed in the epithelial cells of the proximal tubules of the kidneys and the stomach, as well as in hematopoietic cells of the bone marrow and spleen. In humans, this gene is expressed in the epithelial cells of the proximal tubules of the kidneys and is present in keratinocytes of the basal layer of the epidermis.

Subunit：Interacts with YAP1 and CSF1R (By similarity). Interacts with CTNND1; this interaction allows YES1-mediated activation of FYN and FER and subsequent phosphorylation of CTNND1 (By similarity). Interacts with FASLG. Interacts with IL6ST/gp130 (PubMed:25731159). Interacts with SCRIB, when YES1 is in a closed conformation; the interaction facilitates YES1 autophosphorylation (PubMed:33730553)

Synonyms

YES1; YES; Tyrosine-protein kinase Yes; Proto-oncogene c-Yes; p61-Yes

Documentation

User Guide for Antibodies (1077 KB)