XAF1 Antibody (YA6968)

Cat. No.: HY-P87285: Data Sheet User Guide for Antibodies
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XAF1 Antibody (YA6968) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to XAF1.

For research use only. We do not sell to patients.

Size	Price	Stock
20 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

XAF1 Antibody (YA6968) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to XAF1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 35 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q6GPH4

Immunogen

Amino acids 1-225 mapping at the N-terminus of XAF1 of human origin.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:100-1:1000
IP IP: Immunoprecipitation	1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:500

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

Storage & Stability

Stored at 2-8°C for 1 year, do not freeze.

Shipping

Shipping with blue ice.

Background

Function：Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondria, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells

Subcellular Localization：Cytoplasm,Nucleus,Mitochondrion,Nucleus,Nucleus

Expression：
Tissue_Specificity: Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level)
Induction: Up-regulated by IFNB1/IFN-beta in cell lines sensitive to the proapoptotic effects of IFNB1 but not in apoptosis-resistant cells. Up-regulated by TNF in trophoblast cells

Isoforms & Post-Translational Modification：Q6GPH4 has 7 isomers: Q6GPH4-1: 34626 Da (predicted); Q6GPH4-2: 32471 Da (predicted); Q6GPH4-3: 16322 Da (predicted); Q6GPH4-4: 14167 Da (predicted); Q6GPH4-5: 18960 Da (predicted); Q6GPH4-6: 22507 Da (predicted); Q6GPH4-7: 34543 Da (predicted).

Subunit：Interacts with BIRC4; the interaction is not detected in (PubMed:16432762)

Synonyms

BIRC 4 binding protein antibody; BIRC4 binding protein antibody; BIRC4-binding protein antibody; BIRC4BP antibody; HSXIAPAF 1 antibody; HSXIAPAF1 antibody; XAF 1 antibody; XAF1 antibody; XAF1_HUMAN antibody; XIAP associated factor 1 antibody

Documentation

User Guide for Antibodies (1077 KB)