XAF1 Antibody (YA6968)
(Synonyms: BIRC 4 binding protein antibody; BIRC4 binding protein antibody; BIRC4-binding protein antibody; BIRC4BP antibody; HSXIAPAF 1 antibody; HSXIAPAF1 antibody; XAF 1 antibody; XAF1 antibody; XAF1_HUMAN antibody; XIAP associated factor 1 antibody)
Based on 1 Customer Validation
XAF1 Antibody (YA6968) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to XAF1.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, IP
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Reactivity :
Human
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Formulation:
Supplied in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
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| Dilution Ratio | 1:100-1:1000 | 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) | 1:50-1:500 |
Product Details
XAF1 Antibody (YA6968) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to XAF1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 35 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 35 kDa
Amino acids 1-225 mapping at the N-terminus of XAF1 of human origin.
Endogenous
Protein A affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
XAF1 is a Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondria, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells
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Subcellular Localization
Cytoplasm,Nucleus,Mitochondrion,Nucleus,Nucleus
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Expression
Tissue_Specificity: Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level)
Induction: Up-regulated by IFNB1/IFN-beta in cell lines sensitive to the proapoptotic effects of IFNB1 but not in apoptosis-resistant cells. Up-regulated by TNF in trophoblast cells -
Isoforms & Post-Translational Modification
Q6GPH4 has 7 isomers: Q6GPH4-1: 34626 Da (predicted); Q6GPH4-2: 32471 Da (predicted); Q6GPH4-3: 16322 Da (predicted); Q6GPH4-4: 14167 Da (predicted); Q6GPH4-5: 18960 Da (predicted); Q6GPH4-6: 22507 Da (predicted); Q6GPH4-7: 34543 Da (predicted).
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Subunit
Interacts with BIRC4; the interaction is not detected in (PubMed:16432762)
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SwissProt ID
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Synonyms
BIRC 4 binding protein antibody; BIRC4 binding protein antibody; BIRC4-binding protein antibody; BIRC4BP antibody; HSXIAPAF 1 antibody; HSXIAPAF1 antibody; XAF 1 antibody; XAF1 antibody; XAF1_HUMAN antibody; XIAP associated factor 1 antibody
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