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VE Cadherin Antibody (YA6053)

(Synonyms: CDH5; Cadherin-5; 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD antigen CD144)
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Based on 1 Customer Validation

VE Cadherin Antibody (YA6053) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VE Cadherin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for VE Cadherin Antibody (YA6053)
  • VE Cadherin Antibody (YA6053) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

VE Cadherin Antibody (YA6053) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VE Cadherin.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 90-140 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 88 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719131

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for VE Cadherin Antibody (YA6053)
    Western blot analysis of extracts from Rat Lung(lane 2(20μg), Mouse Liver(lane 3(20μg), Mouse Lung(lane 4(20μg) and Mouse Heart(lane 5(20μg) using VE Cadherin Antibody (HY-P86361). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    VE Cadherin is a Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1. Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Associates with CTNND1/p120-catenin to control CADH5 endocytosis[1][2][3].

  • Subcellular Localization

    Cell junction, adherens junction; Cell membrane; Single-pass type I membrane protein; Cytoplasm

  • Expression


    Tissue_specificity:Expression in endothelial cells (protein level) (PubMed: 27338829) . Expression in the brain (PubMed: 2059658) .

  • Isoforms & Post-Translational Modification

    P33151 has 2 isomers: P33151-1: 87528 Da (predicted); P33151-2: 74499 Da (predicted).
    Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB (By similarity);O-glycosylated

  • Subunit

    Part of a complex composed of AMOTL2, MAGI1 and CDH5, within the complex AMOTL2 acts as a scaffold protein for the interaction of MAGI1 with CDH5 (By similarity). The complex is required for coupling actin fibers to cell junctions in endothelial cells (By similarity). Within the complex AMOTL2 (via its N-terminus) interacts with CDH5 (By similarity). Interacts (via cadherin 5 domain) with PTPRB (By similarity). Interacts with TRPC4 (PubMed:19996314). Interacts with KRIT1 (PubMed:20332120). Interacts with PARD3 (By similarity). Interacts with RTN4 (isoform B) (PubMed:21183689). Interacts with PALS1; the interaction promotes PALS1 localization to cell junctions and is required for CDH5-mediated vascular lumen formation and endothelial cell (PubMed:27466317). Interacts with CTNND1/p120-catenin; the interaction controls CADH5 endocytosis (By similarity)

  • SwissProt ID

    P33151

  • Gene ID
    1003 [NCBI]
  • Synonyms

    CDH5; Cadherin-5; 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD antigen CD144

VE Cadherin Antibody (YA6053) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Rat Lung(lane 2(20μg), Mouse Liver(lane 3(20μg), Mouse Lung(lane 4(20μg) and Mouse Heart(lane 5(20μg) using VE Cadherin Antibody (HY-P86361). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • VE Cadherin Antibody (YA6053) Product Image
    1/16
  • Experimental Validation Results for VE Cadherin Antibody (YA6053)
  • VE Cadherin Antibody (YA6053) Product Image
  • Experimental Validation Results for VE Cadherin Antibody (YA6053)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Rat Lung(lane 2(20μg), Mouse Liver(lane 3(20μg), Mouse Lung(lane 4(20μg) and Mouse Heart(lane 5(20μg) using VE Cadherin Antibody (HY-P86361). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for VE Cadherin Antibody (YA6053)
  • VE Cadherin Antibody (YA6053) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Rat Lung(lane 2(20μg), Mouse Liver(lane 3(20μg), Mouse Lung(lane 4(20μg) and Mouse Heart(lane 5(20μg) using VE Cadherin Antibody (HY-P86361). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • VE Cadherin Antibody (YA6053) Product Image WB