FAM120A Antibody (YA10080)
(Synonyms: C9orf10, KIAA0183, OSSA, FAM120A, Constitutive coactivator of PPAR-gamma-like protein 1, Oxidative stress-associated SRC activator, Protein FAM120A)FAM120A Antibody (YA10080) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to FAM120A.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:10-50 |
Product Details
FAM120A Antibody (YA10080) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to FAM120A.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 132 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 121 kDa
Purified recombinant protein of human FAM120A.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
FAM120A is a Membrane-associated scaffold protein that participates in multiple cellular processes, including oxidative stress-induced survival signaling, RNA trafficking and translational regulation, and the modulation of membrane signaling through its interaction with RNA-binding proteins and lipid raft-associated domains. Functions as a regulator of activation of several intracellular kinases, including SRC, FAK, and PI3K, following oxidative stress. Promotes RNA splicing and stability of lipogenic enzymes. Mechanistically, recruits the lipogenic transcription factor SREBP1 at active promoters, thereby bridging the newly transcribed lipogenic genes from RNA polymerase II to the SRSF1 and U1-70K-containing RNA-splicing machinery. Associates with key proteins of the NLRP3 inflammasome pathway and subsequently inhibits NLRP3 inflammasome activation. Sequesters a subset of AGO2-bound mRNAs from miRNA-mediated silencing by binding to poly(G)-rich motifs in their 3'-UTR independently of the AGO2 binding site, likely by preventing recruitment of miRISC effectors (By similarity)[1][2][3].
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Subcellular Localization
Cytoplasm; Cell membrane
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Expression
Tissue_Specificity: Widely expressed. In gastric mucosa, detected in the bottom region of the foveolar epithelium (at protein level). -
Isoforms & Post-Translational Modification
FAM120A has 5 isoforms, Q9NZB2-1: amino acid length is 1118, molecular weight is 121888 Da (predicted); Q9NZB2-2: amino acid length is 628, molecular weight is 68993 Da (predicted); Q9NZB2-4: amino acid length is 1072, molecular weight is 117406 Da (predicted); Q9NZB2-5: amino acid length is 651, molecular weight is 71561 Da (predicted); Q9NZB2-6: amino acid length is 1146, molecular weight is 125272 Da (predicted).Arg-982 is dimethylated, probably to asymmetric dimethylarginine
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Subunit
Interacts with PURA (By similarity).
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SwissProt ID
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Synonyms
C9orf10, KIAA0183, OSSA, FAM120A, Constitutive coactivator of PPAR-gamma-like protein 1, Oxidative stress-associated SRC activator, Protein FAM120A
Documentation
References
[1]. Tanaka M, et al. A novel RNA-binding protein, Ossa/C9orf10, regulates activity of Src kinases to protect cells from oxidative stress-induced apoptosis. Mol Cell Biol. 2009 Jan;29(2):402-13. [Content Brief]
[2]. Cho S, et al. FAM120A couples SREBP-dependent transcription and splicing of lipogenesis enzymes downstream of mTORC1. Mol Cell. 2023 Aug 17;83(16):3010-3026.e8. [Content Brief]
[3]. Yang C, et al. Thermal Proteome Profiling Reveals Meltome Upon NLRP3 Inflammasome Activation. Mol Cell Proteomics. 2025 May;24(5):100972. [Content Brief]
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