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ABL2 Antibody (YA3203)

(Synonyms: ABL2; ABLL; ARG; Abelson tyrosine-protein kinase 2; Abelson murine leukemia viral oncogene homolog 2; Abelson-related gene protein; Tyrosine-protein kinase ARG)
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Based on 1 Customer Validation

ABL2 Antibody (YA3203) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ABL2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ABL2 Antibody (YA3203)
  • Experimental Validation Results for ABL2 Antibody (YA3203)
  • ABL2 Antibody (YA3203) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

ABL2 Antibody (YA3203) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ABL2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 128 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 128 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human ABL2

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105569

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ABL2 Antibody (YA3203)
    Western blot analysis of extracts from Hela(lane 2(20ug) , K562(lane 3(20ug)and Jurkat(lane 4(20ug) using ABL2 Antibody (HY-P83458) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ABL2 Antibody (YA3203)
    Flow cytometric analysis of 1X10^6 Raji cells labeling ABL2 Antibody (HY-P83458, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    ABL2 is a non-receptor tyrosine protein kinase that plays overlapping roles with ABL1 in key processes of cell growth and survival, such as cytoskeleton remodeling in response to extracellular stimuli, cell movement and adhesion, and receptor endocytosis. ABL2 coordinates actin remodeling by tyrosine phosphorylation of proteins that control cytoskeleton dynamics, such as MYH10 (involved in movement), CTTN (involved in signal transduction), or TUBA1 and TUBB (microtubule subunits). It directly binds to F-actin and regulates actin cytoskeleton structure through its F-actin bundle activity. ABL2 participates in the regulation of cell adhesion and movement by phosphorylating key regulators such as CRK, CRKL, DOK1, or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its binding to RASA1, thereby recruiting ARHGAP35 to the cell periphery, where it inhibits RHO. ABL2 phosphorylates multiple receptor tyrosine kinases, such as PDGFRB, and other substrates involved in the regulation of endocytosis, such as RIN1.

  • Subcellular Localization

    Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity:Broad expression

  • Isoforms & Post-Translational Modification

    P42684 has 9 isomers: P42684-1: 128343 Da (predicted); P42684-2: 124560 Da (predicted); P42684-3: 126684 Da (predicted); P42684-4: 114159 Da (predicted); P42684-5: 117942 Da (predicted); P42684-6: 126219 Da (predicted); P42684-7: 115817 Da (predicted); P42684-10: 116283 Da (predicted); P42684-8: 60864 Da (predicted).
    Phosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB (By similarity);Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation

  • Subunit

    Interacts with PSMA7. Interacts with CTTN. Found in a complex with ABL1, ABL2, CRK and UNC119; leading to the inhibition of CRK phosphorylation by ABL kinases

  • SwissProt ID

    P42684

  • Gene ID
    27 [NCBI]
  • Synonyms

    ABL2; ABLL; ARG; Abelson tyrosine-protein kinase 2; Abelson murine leukemia viral oncogene homolog 2; Abelson-related gene protein; Tyrosine-protein kinase ARG

  • Research Field

    Signal Transduction

ABL2 Antibody (YA3203) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , K562(lane 3(20ug)and Jurkat(lane 4(20ug) using ABL2 Antibody (HY-P83458) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Raji cells labeling ABL2 Antibody (HY-P83458, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ABL2 Antibody (YA3203) Product Image
    1/16
  • Experimental Validation Results for ABL2 Antibody (YA3203)
  • Experimental Validation Results for ABL2 Antibody (YA3203)
  • ABL2 Antibody (YA3203) Product Image
  • Experimental Validation Results for ABL2 Antibody (YA3203)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , K562(lane 3(20ug)and Jurkat(lane 4(20ug) using ABL2 Antibody (HY-P83458) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ABL2 Antibody (YA3203)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Raji cells labeling ABL2 Antibody (HY-P83458, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for ABL2 Antibody (YA3203)
  • Experimental Validation Results for ABL2 Antibody (YA3203)
  • ABL2 Antibody (YA3203) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) , K562(lane 3(20ug)and Jurkat(lane 4(20ug) using ABL2 Antibody (HY-P83458) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ABL2 Antibody (YA3203) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 Raji cells labeling ABL2 Antibody (HY-P83458, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ABL2 Antibody (YA3203) Product Image WB
  • ABL2 Antibody (YA3203) Product Image FC