HELIC1 Antibody
(Synonyms: HELIC1, RQT2, ASCC3, Activating signal cointegrator 1 complex subunit 3, ASC-1 complex subunit p200, Trip4 complex subunit p200, ASC1p200)HELIC1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to HELIC1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
HELIC1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to HELIC1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 155kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 13; 83; 251 kDa
Purified recombinant protein of human HELIC1
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
HELIC1 is an ATPase involved both in DNA repair and rescue of stalled ribosomes. 3'-5' DNA helicase involved in repair of alkylated DNA: promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3, enabling ALKBH3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions. Also involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation. Drives the splitting of stalled ribosomes that are ubiquitinated in a ZNF598-dependent manner, as part of the ribosome quality control trigger (RQT) complex. Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation[1][2][3][4][5][6].
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Subcellular Localization
Nucleus; Nucleus speckle; Cytoplasm, cytosol
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Expression
Tissue_Specificity: Ubiquitous. -
Isoforms & Post-Translational Modification
HELIC1 has 3 isoforms, Q8N3C0-1: amino acid length is 2202, molecular weight is 251460 Da (predicted); Q8N3C0-3: amino acid length is 111, molecular weight is 13014 Da (predicted); Q8N3C0-4: amino acid length is 731, molecular weight is 83675 Da (predicted).
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Subunit
Identified in the ASCC complex that contains ASCC1, ASCC2 and ASCC3.
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SwissProt ID
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Synonyms
HELIC1, RQT2, ASCC3, Activating signal cointegrator 1 complex subunit 3, ASC-1 complex subunit p200, Trip4 complex subunit p200, ASC1p200
Documentation
[1]. Dango S, et al. DNA unwinding by ASCC3 helicase is coupled to ALKBH3-dependent DNA alkylation repair and cancer cell proliferation. Mol Cell. 2011 Nov 4;44(3):373-84. [Content Brief]
[2]. Matsuo Y, et al. Ubiquitination of stalled ribosome triggers ribosome-associated quality control. Nat Commun. 2017 Jul 31;8(1):159. [Content Brief]
[3]. Hashimoto S, et al. Identification of a novel trigger complex that facilitates ribosome-associated quality control in mammalian cells. Sci Rep. 2020 Feb 25;10(1):3422. [Content Brief]
[4]. Juszkiewicz S, et al. The ASC-1 Complex Disassembles Collided Ribosomes. Mol Cell. 2020 Aug 20;79(4):603-614.e8. [Content Brief]
[5]. Narita M, et al. A distinct mammalian disome collision interface harbors K63-linked polyubiquitination of uS10 to trigger hRQT-mediated subunit dissociation. Nat Commun. 2022 Oct 27;13(1):6411. [Content Brief]
[6]. Jung DJ, et al. Novel transcription coactivator complex containing activating signal cointegrator 1. Mol Cell Biol. 2002 Jul;22(14):5203-11. [Content Brief]
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