FER Antibody (YA4850)

Cat. No.: HY-P85158: Data Sheet User Guide for Antibodies
FAQs
Technical Support

FER Antibody (YA4850) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to FER.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

FER Antibody (YA4850) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to FER.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 95 kDa;

Species Reactivity

Human, Mouse

SwissProt ID

P16591

Gene ID

2241 [NCBI]

Immunogen

Purified recombinant fragment of human Fer expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Plays a role in synapse organization, trafficking of synaptic vesicles, the generation of excitatory postsynaptic currents and neuron-neuron synaptic transmission. Plays a role in neuronal cell death after brain damage. Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3, but the biological relevance of this depends on cell type and stimulus

Subcellular Localization：Cytoplasm; Cytoplasm, cytoskeleton; Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection; Cell junction; Membrane; Peripheral membrane protein; Cytoplasmic side; Nucleus; Cytoplasm, cell cortex

Expression：
Tissue_specificity:Isoform 1 is detected in normal colon and in fibroblasts (at protein level) . Isoform 3 is detected in normal testis, in colon carcinoma-derived metastases in lung, liver and ovary, and in colon carcinoma and hepato carcinoma cell lines (at protein level) . Isoform 3 is not detected in normal colon or in normal fibroblasts (at protein level) . Widely expressed

Isoforms & Post-Translational Modification：P16591 has 3 isomers: P16591-1: 94638 Da (predicted); P16591-2: 74266 Da (predicted); P16591-3: 51643 Da (predicted).
Autophosphorylated;Polyubiquitinated; this leads to proteasomal degradation

Subunit：Homotrimer. Interacts with ARHGDIA, IRS1, JAK1, NRP1, PIK3R1, PLEC and TMF1.

Synonyms

FER; TYK3; Tyrosine-protein kinase Fer; Feline encephalitis virus-related kinase FER; Fujinami poultry sarcoma/Feline sarcoma-related protein Fer; Proto-oncogene c-Fer; Tyrosine kinase 3; p94-Fer

Documentation

User Guide for Antibodies (1077 KB)