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UBE2D3 Antibody (YA2333)

(Synonyms: Ubiquitin Conjugating Enzyme E2 D3; UBE2D3; UBC4/5; UBCH5C; E2(17)KB3)
Customer Review

Based on 1 Customer Validation

Ubiquitin Conjugating Enzyme E2 D3 Antibody (YA2333) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Conjugating Enzyme E2 D3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for UBE2D3 Antibody (YA2333)
  • Experimental Validation Results for UBE2D3 Antibody (YA2333)
  • UBE2D3 Antibody (YA2333) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Ubiquitin Conjugating Enzyme E2 D3 Antibody (YA2333) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Conjugating Enzyme E2 D3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 17 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 17 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human UBE2D3

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104689

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for UBE2D3 Antibody (YA2333)
    Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), 293T (lane 3, 15 μg), Hela (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using Ubiquitin Conjugating Enzyme E2 D3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for UBE2D3 Antibody (YA2333)
    Western blot analysis of extracts from C6(lane 2(20ug) , NIH/3T3(lane 3(20ug) and Hela(lane 4(20ug) using Ubiquitin Conjugating Enzyme E2 D3 Antibody (HY-P82588) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    UBE2D3 is an Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Also acts as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction. Together with RNF135, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production. Together with ZNF598, catalyzes ubiquitination of 40S ribosomal proteins in response to ribosome collisions. In cooperation with the GATOR2 complex, catalyzes 'Lys-6'-linked ubiquitination of NPRL2[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18].

  • Subcellular Localization

    Cell membrane; Peripheral membrane protein; Endosome membrane; Peripheral membrane protein

  • Isoforms & Post-Translational Modification

    P61077 has 3 isomers: P61077-1: 16687 Da (predicted); P61077-2: 16785 Da (predicted); P61077-3: 16893 Da (predicted).
    Phosphorylated by AURKB

  • Subunit

    Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex; when Cullin is neddylated, the interaction between the E2 and the SCF complex is strengthened. Interacts with DAPK3. Interacts with BRCA1; the DNA damage checkpoint promotes the association with BRCA1 after ionizing radiation. Interacts non-covalently with ubiquitin. Interacts with E3 ubiquitin-protein ligase CBLC. Interacts with UBTD1 (PubMed:24211586). Interacts with RIGI and RNF135; involved in RIGI ubiquitination and activation (PubMed:28469175)

  • SwissProt ID

    P61077

  • Gene ID
    7323 [NCBI]
  • Synonyms

    Ubiquitin Conjugating Enzyme E2 D3; UBE2D3; UBC4/5; UBCH5C; E2(17)KB3

  • Research Field

    Epigenetics and Nuclear Signaling

UBE2D3 Antibody (YA2333) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), 293T (lane 3, 15 μg), Hela (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using Ubiquitin Conjugating Enzyme E2 D3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) , NIH/3T3(lane 3(20ug) and Hela(lane 4(20ug) using Ubiquitin Conjugating Enzyme E2 D3 Antibody (HY-P82588) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • UBE2D3 Antibody (YA2333) Product Image
    1/16
  • Experimental Validation Results for UBE2D3 Antibody (YA2333)
  • Experimental Validation Results for UBE2D3 Antibody (YA2333)
  • UBE2D3 Antibody (YA2333) Product Image
  • Experimental Validation Results for UBE2D3 Antibody (YA2333)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), 293T (lane 3, 15 μg), Hela (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using Ubiquitin Conjugating Enzyme E2 D3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for UBE2D3 Antibody (YA2333)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) , NIH/3T3(lane 3(20ug) and Hela(lane 4(20ug) using Ubiquitin Conjugating Enzyme E2 D3 Antibody (HY-P82588) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for UBE2D3 Antibody (YA2333)
  • Experimental Validation Results for UBE2D3 Antibody (YA2333)
  • UBE2D3 Antibody (YA2333) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), 293T (lane 3, 15 μg), Hela (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using Ubiquitin Conjugating Enzyme E2 D3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • UBE2D3 Antibody (YA2333) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6(lane 2(20ug) , NIH/3T3(lane 3(20ug) and Hela(lane 4(20ug) using Ubiquitin Conjugating Enzyme E2 D3 Antibody (HY-P82588) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • UBE2D3 Antibody (YA2333) Product Image WB
  • UBE2D3 Antibody (YA2333) Product Image WB