UBE2D3 Antibody (YA2333)

Art. -Nr.: HY-P82588: Data Sheet COA
SDS
User Guide for Antibodies
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Ubiquitin Conjugating Enzyme E2 D3 Antibody (YA2333) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Conjugating Enzyme E2 D3.

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UBE2D3 Antibody (YA2333) Spezialitätsempfehlungen:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Beschreibung

Beschreibung

Ubiquitin Conjugating Enzyme E2 D3 Antibody (YA2333) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Conjugating Enzyme E2 D3.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molekulargewicht

Predicted band size: 17 kDa;

Observed band size: 17 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P61077

Gene ID

7323 [NCBI]

Immunogen

A synthetic peptide of human UBE2D3

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Reinheit	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), 293T (lane 3, 15 μg), Hela (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using Ubiquitin Conjugating Enzyme E2 D3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis of extracts from C6(lane 2(20ug) , NIH/3T3(lane 3(20ug) and Hela(lane 4(20ug) using Ubiquitin Conjugating Enzyme E2 D3 Antibody (HY-P82588) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:15247280, PubMed:15496420, PubMed:18284575, PubMed:20061386, PubMed:21532592, PubMed:28322253). In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination (PubMed:15247280, PubMed:15496420, PubMed:18284575, PubMed:20061386, PubMed:21532592). Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation (PubMed:20347421). Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin (PubMed:10329681). Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin (PubMed:10329681). Also acts as an initiator E2, in conjunction with RNF8, for the priming of PCNA (PubMed:18948756). Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (PubMed:18948756). Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair (PubMed:16628214). Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus (PubMed:18515077). In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53 (PubMed:12646252, PubMed:15280377). In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes (PubMed:18508924). In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction (PubMed:11743028). Together with RNF135, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production (PubMed:28469175). Together with ZNF598, catalyzes ubiquitination of 40S ribosomal proteins in response to ribosome collisions (PubMed:28685749). In cooperation with the GATOR2 complex, catalyzes 'Lys-6'-linked ubiquitination of NPRL2 (PubMed:36528027)

Subcellular Localization：Cell membrane; Peripheral membrane protein; Endosome membrane; Peripheral membrane protein

Isoforms & Post-Translational Modification：P61077 has 3 isomers: P61077-1: 16687 Da (predicted); P61077-2: 16785 Da (predicted); P61077-3: 16893 Da (predicted).
Phosphorylated by AURKB

Subunit：Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex; when Cullin is neddylated, the interaction between the E2 and the SCF complex is strengthened. Interacts with DAPK3. Interacts with BRCA1; the DNA damage checkpoint promotes the association with BRCA1 after ionizing radiation. Interacts non-covalently with ubiquitin. Interacts with E3 ubiquitin-protein ligase CBLC. Interacts with UBTD1 (PubMed:24211586). Interacts with RIGI and RNF135; involved in RIGI ubiquitination and activation (PubMed:28469175)

RRID

AB_3104689

Database

Entrez Gene: 7323 Human ; 66105 Mouse ; 81920 Rat

SwissProt: P61077 Human ; P61079 Mouse ; P61078 Rat

OMIM: 602963 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

Ubiquitin Conjugating Enzyme E2 D3; UBE2D3; UBC4/5; UBCH5C; E2(17)KB3

Dokumentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

UBE2D3 Antibody (YA2333) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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UBE2D3 Antibody (YA2333)

UBE2D3 Antibody (YA2333) Spezialitätsempfehlungen:

Product Detail

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Background

Beschreibung

UBE2D3 Antibody (YA2333) Related Classifications

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