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EN1 Antibody (YA2055)

(Synonyms: EN1; Engrailed 1; Engrailed; Hu-En-1; Mo-en.1)
Customer Review

Based on 1 Customer Validation

EN1 Antibody (YA2055) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EN1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for EN1 Antibody (YA2055)
  • Experimental Validation Results for EN1 Antibody (YA2055)
  • EN1 Antibody (YA2055) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50 1:50-1:100

Product Details

Description

EN1 Antibody (YA2055) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EN1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 40 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Engrailed 1 aa350-392.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104421

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EN1 Antibody (YA2055)
    Western blot analysis of extracts from HEK293T (lane 2(20μg) and HEK293T (lane 3(40μg) using EN1 (HY-P82310) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EN1 Antibody (YA2055)
    Flow cytometric analysis of 1X10^6 SH-SY5Y cells labeling EN1 Antibody (YA2055) (HY-P82310, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    EN1 is required for proper formation of the apical ectodermal ridge and correct dorsal-ventral patterning in the limb

  • Subcellular Localization

    Nucleus

  • SwissProt ID

    Q05925

  • Gene ID
    2019 [NCBI]
  • Synonyms

    EN1; Engrailed 1; Engrailed; Hu-En-1; Mo-en.1

  • Research Field

    Neuroscience

EN1 Antibody (YA2055) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane 2(20μg) and HEK293T (lane 3(40μg) using EN1 (HY-P82310) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 SH-SY5Y cells labeling EN1 Antibody (YA2055) (HY-P82310, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • EN1 Antibody (YA2055) Product Image
    1/16
  • Experimental Validation Results for EN1 Antibody (YA2055)
  • Experimental Validation Results for EN1 Antibody (YA2055)
  • EN1 Antibody (YA2055) Product Image
  • Experimental Validation Results for EN1 Antibody (YA2055)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane 2(20μg) and HEK293T (lane 3(40μg) using EN1 (HY-P82310) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EN1 Antibody (YA2055)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 SH-SY5Y cells labeling EN1 Antibody (YA2055) (HY-P82310, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for EN1 Antibody (YA2055)
  • Experimental Validation Results for EN1 Antibody (YA2055)
  • EN1 Antibody (YA2055) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T (lane 2(20μg) and HEK293T (lane 3(40μg) using EN1 (HY-P82310) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • EN1 Antibody (YA2055) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 SH-SY5Y cells labeling EN1 Antibody (YA2055) (HY-P82310, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • EN1 Antibody (YA2055) Product Image WB
  • EN1 Antibody (YA2055) Product Image FC