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Claudin 3 Antibody (YA2512)

(Synonyms: RVP1; HRVP1; C7orf1; CPE-R2; CPETR2)
Customer Review

Based on 1 Customer Validation

Claudin 3 Antibody (YA2512) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Claudin 3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Claudin 3 Antibody (YA2512)
  • Experimental Validation Results for Claudin 3 Antibody (YA2512)
  • Claudin 3 Antibody (YA2512) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

Claudin 3 Antibody (YA2512) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Claudin 3.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 20 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Species Reactivity Database

Entrez Gene: 1365 Human

SwissProt: O15551 Human

Immunogen

A synthetic peptide of human Claudin 3

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104041

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Claudin 3 Antibody (YA2512)
    Immunocytochemistry analysis of A431 cells labeling Vitamin D Binding Protein with Vitamin D Binding Protein Antibody (HY-P80930) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vitamin D Binding ProteinAntibody ((HY-P80930) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Claudin 3 Antibody (YA2512)
    Immunocytochemistry analysis of HepG2 cells labeling Vitamin D Binding Proteinwith Vitamin D Binding Protein Antibody (HY-P80930) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vitamin D Binding Protein Antibody ((HY-P80930) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Claudin 3 is a Barrier-forming claudin. Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity

  • Subcellular Localization

    Cell junction, tight junction; Cell membrane; Multi-pass membrane protein

  • Subunit

    Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN1 and CLDN2 homopolymers. Interacts in cis (within the same plasma membrane) with CLDN19 (PubMed:28028216, PubMed:36008380). Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity)

  • SwissProt ID

    O15551

  • Gene ID
    1365 [NCBI]
  • Synonyms

    RVP1; HRVP1; C7orf1; CPE-R2; CPETR2

  • Research Field

    Cell Biology

Claudin 3 Antibody (YA2512) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Vitamin D Binding Protein with Vitamin D Binding Protein Antibody (HY-P80930) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vitamin D Binding ProteinAntibody ((HY-P80930) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Vitamin D Binding Proteinwith Vitamin D Binding Protein Antibody (HY-P80930) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vitamin D Binding Protein Antibody ((HY-P80930) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Claudin 3 Antibody (YA2512) Product Image
    1/16
  • Experimental Validation Results for Claudin 3 Antibody (YA2512)
  • Experimental Validation Results for Claudin 3 Antibody (YA2512)
  • Claudin 3 Antibody (YA2512) Product Image
  • Experimental Validation Results for Claudin 3 Antibody (YA2512)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Vitamin D Binding Protein with Vitamin D Binding Protein Antibody (HY-P80930) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vitamin D Binding ProteinAntibody ((HY-P80930) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Claudin 3 Antibody (YA2512)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Vitamin D Binding Proteinwith Vitamin D Binding Protein Antibody (HY-P80930) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vitamin D Binding Protein Antibody ((HY-P80930) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Claudin 3 Antibody (YA2512)
  • Experimental Validation Results for Claudin 3 Antibody (YA2512)
  • Claudin 3 Antibody (YA2512) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling Vitamin D Binding Protein with Vitamin D Binding Protein Antibody (HY-P80930) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vitamin D Binding ProteinAntibody ((HY-P80930) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Claudin 3 Antibody (YA2512) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Vitamin D Binding Proteinwith Vitamin D Binding Protein Antibody (HY-P80930) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Vitamin D Binding Protein Antibody ((HY-P80930) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Claudin 3 Antibody (YA2512) Product Image ICC
  • Claudin 3 Antibody (YA2512) Product Image ICC