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DYNC1LI1 Antibody (YA7831)

(Synonyms: DLC-A; DNCLI1; LIC1)
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Based on 1 Customer Validation

DYNC1LI1 Antibody (YA7831) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DYNC1LI1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
  • DYNC1LI1 Antibody (YA7831) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-2000 1:100-250 1:100

Product Details

Description

DYNC1LI1 Antibody (YA7831) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DYNC1LI1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 56.4 kDa
Immunogen

Full length human recombinant protein of human DYNC1LI1 produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
    Western blot analysis of extracts from K562(lane 2(20μg) , Hela(lane 3(20μg) ,Jurkat(lane 4(20μg)and Mouse heart tissue( lane 5(20μg) using DYNC1LI1 Antibody (HY-P88147). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
    Immunocytochemistry analysis of Hela cells labeling DYNC1LI1 with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
    Immunocytochemistry analysis of PC-3 cells labeling DYNC1LI1 with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    DYNC1LI1 acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress through the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores. Forms a functional Rab11/RAB11FIP3/dynein complex onto endosomal membrane that regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endosomal recycling compartment (ERC)[1].

  • Subcellular Localization

    Cytoplasm,Chromosome, centromere, kinetochore,Cytoplasm, cytoskeleton, spindle pole,Recycling endosome membrane

  • Isoforms & Post-Translational Modification

    Q9Y6G9: 523 amino acids, molecular weight 56579 Da.

  • Subunit

    Homodimer (By similarity)

  • SwissProt ID

    Q9Y6G9

  • Synonyms

    DLC-A; DNCLI1; LIC1

DYNC1LI1 Antibody (YA7831) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg) , Hela(lane 3(20μg) ,Jurkat(lane 4(20μg)and Mouse heart tissue( lane 5(20μg) using DYNC1LI1 Antibody (HY-P88147). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling DYNC1LI1 with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling DYNC1LI1 with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DYNC1LI1 Antibody (YA7831) Product Image
    1/16
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
  • DYNC1LI1 Antibody (YA7831) Product Image
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg) , Hela(lane 3(20μg) ,Jurkat(lane 4(20μg)and Mouse heart tissue( lane 5(20μg) using DYNC1LI1 Antibody (HY-P88147). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling DYNC1LI1 with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling DYNC1LI1 with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
  • Experimental Validation Results for DYNC1LI1 Antibody (YA7831)
  • DYNC1LI1 Antibody (YA7831) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20μg) , Hela(lane 3(20μg) ,Jurkat(lane 4(20μg)and Mouse heart tissue( lane 5(20μg) using DYNC1LI1 Antibody (HY-P88147). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • DYNC1LI1 Antibody (YA7831) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling DYNC1LI1 with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DYNC1LI1 Antibody (YA7831) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling DYNC1LI1 with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DYNC1LI1 Antibody (HY-P88147) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DYNC1LI1 Antibody (YA7831) Product Image WB
  • DYNC1LI1 Antibody (YA7831) Product Image ICC
  • DYNC1LI1 Antibody (YA7831) Product Image ICC