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EPHA7 Antibody (YA4835)

(Synonyms: EPHA7; EHK3; HEK11; Ephrin type-A receptor 7; EPH homology kinase 3; EHK-3; EPH-like kinase 11; EK11; hEK11)
Customer Review

Based on 1 Customer Validation

EPHA7 Antibody (YA4835) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to EPHA7.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • EPHA7 Antibody (YA4835) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:10000 1:50-200

Product Details

Description

EPHA7 Antibody (YA4835) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to EPHA7.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 112 kDa
Immunogen

Purified recombinant fragment of EphA7 (aa27-210) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

RRID

AB_3719019

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    Western blot analysis of extracts from Hela (lane 2, 20ug) and Hela (lane 3, 40ug) using EPHA7 Antibody (HY-P85143). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    Immunohistochemical analysis of paraffin-embedded human cervix tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    Immunohistochemical analysis of paraffin-embedded human breast tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    EPHA7 receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 and MAPK3 which are phosphorylated upon activation of EPHA7

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Widely expressed

  • Isoforms & Post-Translational Modification

    Q15375 has 5 isomers: Q15375-1: 112097 Da (predicted); Q15375-2: 111504 Da (predicted); Q15375-3: 31833 Da (predicted); Q15375-4: 111623 Da (predicted); Q15375-5: 50521 Da (predicted).
    Phosphorylated

  • Subunit

    Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer.

  • SwissProt ID

    Q15375

  • Gene ID
    2045 [NCBI]
  • Synonyms

    EPHA7; EHK3; HEK11; Ephrin type-A receptor 7; EPH homology kinase 3; EHK-3; EPH-like kinase 11; EK11; hEK11

EPHA7 Antibody (YA4835) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug) and Hela (lane 3, 40ug) using EPHA7 Antibody (HY-P85143). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPHA7 Antibody (YA4835) Product Image
    1/16
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • EPHA7 Antibody (YA4835) Product Image
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug) and Hela (lane 3, 40ug) using EPHA7 Antibody (HY-P85143). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • Experimental Validation Results for EPHA7 Antibody (YA4835)
  • EPHA7 Antibody (YA4835) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2, 20ug) and Hela (lane 3, 40ug) using EPHA7 Antibody (HY-P85143). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • EPHA7 Antibody (YA4835) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervix tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPHA7 Antibody (YA4835) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPHA7 Antibody (YA4835) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPHA7 Antibody (YA4835) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPHA7 Antibody (YA4835) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPHA7 Antibody (YA4835) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast tissue using EPHA7 Antibody (HY-P85143, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPHA7 Antibody (YA4835) Product Image WB
  • EPHA7 Antibody (YA4835) Product Image IHC-P
  • EPHA7 Antibody (YA4835) Product Image IHC-P
  • EPHA7 Antibody (YA4835) Product Image IHC-P
  • EPHA7 Antibody (YA4835) Product Image IHC-P
  • EPHA7 Antibody (YA4835) Product Image IHC-P
  • EPHA7 Antibody (YA4835) Product Image IHC-P