SFT Antibody (YA3238)(PBS only)

Cat. No.: HY-P83493A: Data Sheet User Guide for Antibodies
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SFT Antibody (YA3238) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SFT.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SFT Antibody (YA3238) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SFT.

Host

Rabbit

Molecular Weight

Predicted band size: 17 kDa;

Observed band size: 17 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P51668

Gene ID

7321 [NCBI]

Immunogen

A synthetic peptide of human SFT aa119-132.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
FC FC: Flow Cytometry	1:10-1:100
IP IP: Immunoprecipitation	1:10-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:22496338). In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:20061386). Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1 (PubMed:18042044, PubMed:18359941). Ubiquitinates STUB1-associated HSP90AB1 in vitro (PubMed:18042044). Lacks inherent specificity for any particular lysine residue of ubiquitin (PubMed:18042044). Essential for viral activation of IRF3 (PubMed:19854139). Mediates polyubiquitination of CYP3A4 (PubMed:19103148)

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:Ubiquitous. Up-regulated in livers of iron-overloaded with hereditary hemochromatosis

Subunit：Component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Interacts with RNF11

Research Field

Synonyms

ubiquitin conjugating enzyme E2 D1; SFT; UBCH5; UBC4/5; UBCH5A; E2(17)KB1

Documentation

User Guide for Antibodies (1077 KB)