UBE2D1 - ubiquitin conjugating enzyme E2 D1 Gene

Also Known as SFT; UBCH5; UBC4/5; UBCH5A; E2(17)KB1

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 7321

About UBE2D1

Cytogenetic location: 10q21.1 Genomic coordinates (GRCh38): 10:58,335,006-58,370,748 (from NCBI)

This gene has 4 transcripts (splice variants), 274 orthologues and 12 paralogues. Ubiquitous expression in bone marrow (RPKM 16.4), appendix (RPKM 13.7) and 25 other tissues.

Summary

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating Enzymes, or E1s, ubiquitin-conjugating Enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

UBE2D1 Products (2)

mRNA	Protein	Name
NM_001204880.2	NP_001191809.1	ubiquitin-conjugating enzyme E2 D1 isoform 2
NM_003338.5	NP_003329.1	ubiquitin-conjugating enzyme E2 D1 isoform 1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables protein binding	IPI IPI: Inferred from physical interaction	16189514	GOA
enables ubiquitin conjugating enzyme activity	IDA IDA: Inferred from direct assay	15247280	GOA
enables ubiquitin protein ligase activity	IDA IDA: Inferred from direct assay	8530467	GOA
enables ubiquitin-protein transferase activity	IDA IDA: Inferred from direct assay	18359941	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in negative regulation of TORC1 signaling	IDA IDA: Inferred from direct assay	35927303	GOA
involved in positive regulation of protein ubiquitination	IDA IDA: Inferred from direct assay	16275645	GOA
involved in proteasome-mediated ubiquitin-dependent protein catabolic process	IDA IDA: Inferred from direct assay	35927303	GOA
involved in protein K48-linked ubiquitination	IDA IDA: Inferred from direct assay	20061386	GOA
involved in protein polyubiquitination	IDA IDA: Inferred from direct assay	15247280	GOA
involved in ubiquitin-dependent protein catabolic process	IDA IDA: Inferred from direct assay	16275645	GOA

Cellular Component GO Annotation	Evidence	References	Source
located in cytoplasm	IDA IDA: Inferred from direct assay	18845142	GOA
part of protein-containing complex	IDA IDA: Inferred from direct assay	22797923	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

UBE2D1 Protein Structure

UQ_con

0
100
147 a.a.

Protein Preferred Names	Protein Names
ubiquitin-conjugating enzyme E2 D1	(E3-independent) E2 ubiquitin-conjugating enzyme D1

UBE2D1 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	UBE2D1	P51668	MID2	Homo sapiens	Q9UJV3-2	Y2H Prey Pooling	25416956
Intra	UBE2D1	P51668	MID2	Homo sapiens	Q9UJV3-2	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	MID2	Homo sapiens	Q9UJV3-2	Validated Y2H	32296183
Intra	UBE2D1	P51668	MID2	Homo sapiens	Q9UJV3-2	Y2H Array	25416956
Intra	UBE2D1	P51668	MID2	Homo sapiens	Q9UJV3-2	Y2H Array	32296183
Intra	UBE2D1	P51668	OTUB1	Homo sapiens	Q96FW1	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	OTUB1	Homo sapiens	Q96FW1	Y2H	19549727
Intra	UBE2D1	P51668	OTUB1	Homo sapiens	Q96FW1	Anti Tag CoIP	22124327
Intra	UBE2D1	P51668	OTUB1	Homo sapiens	Q96FW1	Y2H Array	32296183
Intra	UBE2D1	P51668	OTUB1	Homo sapiens	Q96FW1	Anti Tag CoIP	33961781
Intra	UBE2D1	P51668	OTUB1	Homo sapiens	Q96FW1	Validated Y2H	32296183
Intra	UBE2D1	P51668	TRIM39	Homo sapiens	Q9HCM9-2	Validated Y2H	32296183
Intra	UBE2D1	P51668	TRIM39	Homo sapiens	Q9HCM9-2	Y2H Array	32296183
Intra	UBE2D1	P51668	TRIM39	Homo sapiens	Q9HCM9-2	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	CHFR	Homo sapiens	Q96EP1-2	Validated Y2H	32296183
Intra	UBE2D1	P51668	CHFR	Homo sapiens	Q96EP1-2	Y2H Array	32296183
Intra	UBE2D1	P51668	CHFR	Homo sapiens	Q96EP1-2	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	MEOX2	Homo sapiens	Q6FHY5	Y2H Array	32296183
Intra	UBE2D1	P51668	MEOX2	Homo sapiens	Q6FHY5	Validated Y2H	32296183
Intra	UBE2D1	P51668	MEOX2	Homo sapiens	Q6FHY5	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	RNF181	Homo sapiens	Q9P0P0	Y2H Array	32296183
Intra	UBE2D1	P51668	RNF181	Homo sapiens	Q9P0P0	Y2H Array	19690564
Intra	UBE2D1	P51668	RNF181	Homo sapiens	Q9P0P0	Validated Y2H	32296183
Intra	UBE2D1	P51668	RNF181	Homo sapiens	Q9P0P0	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	RNF111	Homo sapiens	Q6ZNA4	Y2H Array	19549727
Intra	UBE2D1	P51668	RNF111	Homo sapiens	Q6ZNA4	Y2H Array	25416956
Intra	UBE2D1	P51668	RNF111	Homo sapiens	Q6ZNA4	Y2H Prey Pooling	25416956
Intra	UBE2D1	P51668	RNF25	Homo sapiens	Q96BH1	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	RNF25	Homo sapiens	Q96BH1	Validated Y2H	32296183
Intra	UBE2D1	P51668	RNF25	Homo sapiens	Q96BH1	Anti Tag CoIP	33961781
Intra	UBE2D1	P51668	RNF25	Homo sapiens	Q96BH1	Y2H Array	32296183
Intra	UBE2D1	P51668	RNF25	Homo sapiens	Q96BH1	Y2H Array	19690564
Intra	UBE2D1	P51668	RNF115	Homo sapiens	Q9Y4L5	Y2H Array	32296183
Intra	UBE2D1	P51668	RNF115	Homo sapiens	Q9Y4L5	Y2H Array	19690564
Intra	UBE2D1	P51668	RNF115	Homo sapiens	Q9Y4L5	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	RNF115	Homo sapiens	Q9Y4L5	Y2H Array	31515488
Intra	UBE2D1	P51668	RNF115	Homo sapiens	Q9Y4L5	Validated Y2H	25416956
Intra	UBE2D1	P51668	RNF115	Homo sapiens	Q9Y4L5	Validated Y2H	32296183
Intra	UBE2D1	P51668	ZNRF1	Homo sapiens	Q8ND25	Y2H Array	19690564
Intra	UBE2D1	P51668	ZNRF1	Homo sapiens	Q8ND25	Validated Y2H	32296183
Intra	UBE2D1	P51668	ZNRF1	Homo sapiens	Q8ND25	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	ZNRF1	Homo sapiens	Q8ND25	Y2H Array	32296183
Intra	UBE2D1	P51668	TRIM3	Homo sapiens	O75382	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	TRIM3	Homo sapiens	O75382	Y2H Array	32296183
Intra	UBE2D1	P51668	RNF182	Homo sapiens	Q8N6D2	Y2H Array	32296183
Intra	UBE2D1	P51668	RNF182	Homo sapiens	Q8N6D2	Validated Y2H	32296183
Intra	UBE2D1	P51668	RNF182	Homo sapiens	Q8N6D2	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	RNF14	Homo sapiens	Q9UBS8	Y2H Array	25416956
Intra	UBE2D1	P51668	RNF14	Homo sapiens	Q9UBS8	Y2H Prey Pooling	25416956
Intra	UBE2D1	P51668	TRIM54	Homo sapiens	Q9BYV2	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	TRIM54	Homo sapiens	Q9BYV2	Validated Y2H	32296183
Intra	UBE2D1	P51668	TRIM54	Homo sapiens	Q9BYV2	Y2H Array	32296183
Intra	UBE2D1	P51668	RNF185	Homo sapiens	Q96GF1	Validated Y2H	32296183
Intra	UBE2D1	P51668	RNF185	Homo sapiens	Q96GF1	Y2H Array	32296183
Intra	UBE2D1	P51668	RNF185	Homo sapiens	Q96GF1	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	DTX3	Homo sapiens	Q8N9I9	Validated Y2H	32296183
Intra	UBE2D1	P51668	DTX3	Homo sapiens	Q8N9I9	Y2H Array	32296183
Intra	UBE2D1	P51668	DTX3	Homo sapiens	Q8N9I9	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	MKRN3	Homo sapiens	Q13064	Validated Y2H	25416956
Intra	UBE2D1	P51668	MKRN3	Homo sapiens	Q13064	Y2H Array	25416956
Intra	UBE2D1	P51668	MID1	Homo sapiens	O15344	Y2H Array	32296183
Intra	UBE2D1	P51668	MID1	Homo sapiens	O15344	Validated Y2H	25416956
Intra	UBE2D1	P51668	MID1	Homo sapiens	O15344	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	MID1	Homo sapiens	O15344	Y2H Array	25416956
Intra	UBE2D1	P51668	TRIM8	Homo sapiens	Q9BZR9	Y2H Array	19549727
Intra	UBE2D1	P51668	TRIM8	Homo sapiens	Q9BZR9	Validated Y2H	32296183
Intra	UBE2D1	P51668	TRIM8	Homo sapiens	Q9BZR9	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	TRIM8	Homo sapiens	Q9BZR9	Y2H Array	32296183
Intra	UBE2D1	P51668	MKRN2	Homo sapiens	Q9H000	Y2H Prey Pooling	25416956
Intra	UBE2D1	P51668	MKRN2	Homo sapiens	Q9H000	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	MKRN2	Homo sapiens	Q9H000	Validated Y2H	32296183
Intra	UBE2D1	P51668	MKRN2	Homo sapiens	Q9H000	Y2H Array	32296183
Intra	UBE2D1	P51668	ARRDC3	Homo sapiens	Q96B67	Validated Y2H	32296183
Intra	UBE2D1	P51668	ARRDC3	Homo sapiens	Q96B67	Y2H Array	32296183
Intra	UBE2D1	P51668	ARRDC3	Homo sapiens	Q96B67	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	RNF5	Homo sapiens	Q99942	Validated Y2H	32296183
Intra	UBE2D1	P51668	RNF5	Homo sapiens	Q99942	Y2H Array	32296183
Intra	UBE2D1	P51668	RNF5	Homo sapiens	Q99942	Y2H Prey Pooling	25416956
Intra	UBE2D1	P51668	RNF5	Homo sapiens	Q99942	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	VCP	Homo sapiens	P55072	Y2H Pooling	32814053
Intra	UBE2D1	P51668	VCP	Homo sapiens	P55072	Validated Y2H	32814053
Intra	UBE2D1	P51668	VCP	Homo sapiens	P55072	Y2H Array	32814053
Intra	UBE2D1	P51668	RNF126	Homo sapiens	Q9BV68	Y2H	19549727
Intra	UBE2D1	P51668	RNF126	Homo sapiens	Q9BV68	Y2H Pooling	16189514
Intra	UBE2D1	P51668	RNF126	Homo sapiens	Q9BV68	Y2H Array	25416956
Intra	UBE2D1	P51668	RNF126	Homo sapiens	Q9BV68	Y2H Prey Pooling	25416956
Intra	UBE2D1	P51668	RNF126	Homo sapiens	Q9BV68	Y2H Array	31515488
Intra	UBE2D1	P51668	RNF126	Homo sapiens	Q9BV68	Y2H Array	19549727
Intra	UBE2D1	P51668	TRAF6	Homo sapiens	Q9Y4K3	Enzymatic Study	19549727
Intra	UBE2D1	P51668	MKRN1	Homo sapiens	Q9UHC7	Y2H Array	32296183
Intra	UBE2D1	P51668	MKRN1	Homo sapiens	Q9UHC7	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	MKRN1	Homo sapiens	Q9UHC7	Validated Y2H	32296183
Intra	UBE2D1	P51668	RNF11	Homo sapiens	Q9Y3C5	Enzymatic Study	19549727
Intra	UBE2D1	P51668	RNF11	Homo sapiens	Q9Y3C5	Y2H Array	19549727
Intra	UBE2D1	P51668	RNF11	Homo sapiens	Q9Y3C5	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	RNF11	Homo sapiens	Q9Y3C5	Y2H	19549727
Intra	UBE2D1	P51668	RNF11	Homo sapiens	Q9Y3C5	Validated Y2H	32296183
Intra	UBE2D1	P51668	RNF11	Homo sapiens	Q9Y3C5	Y2H Array	32296183
Intra	UBE2D1	P51668	XIAP	Homo sapiens	P98170	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	XIAP	Homo sapiens	P98170	Pull Down	17318174
Intra	UBE2D1	P51668	XIAP	Homo sapiens	P98170	Validated Y2H	32296183
Intra	UBE2D1	P51668	XIAP	Homo sapiens	P98170	Y2H Array	32296183
Intra	UBE2D1	P51668	XIAP	Homo sapiens	P98170	Enzymatic Study	19549727
Intra	UBE2D1	P51668	TRIM62	Homo sapiens	Q9BVG3	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	TRIM62	Homo sapiens	Q9BVG3	Validated Y2H	32296183
Intra	UBE2D1	P51668	TRIM62	Homo sapiens	Q9BVG3	Y2H Array	32296183
Intra	UBE2D1	P51668	RNF10	Homo sapiens	Q8N5U6	Anti Tag CoIP	33961781
Intra	UBE2D1	P51668	RNF2	Homo sapiens	Q99496	Y2H Array	19549727
Intra	UBE2D1	P51668	RNF2	Homo sapiens	Q99496	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	RNF2	Homo sapiens	Q99496	Validated Y2H	32296183
Intra	UBE2D1	P51668	RNF2	Homo sapiens	Q99496	Y2H Array	32296183
Intra	UBE2D1	P51668	RNF114	Homo sapiens	Q9Y508	Anti Tag CoIP	33961781
Intra	UBE2D1	P51668	TRIM39	Homo sapiens	Q9HCM9	Validated Y2H	25416956
Intra	UBE2D1	P51668	TRIM39	Homo sapiens	Q9HCM9	Y2H Pooling	16189514
Intra	UBE2D1	P51668	DTX2	Homo sapiens	Q86UW9	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	DTX2	Homo sapiens	Q86UW9	Validated Y2H	32296183
Intra	UBE2D1	P51668	DTX2	Homo sapiens	Q86UW9	Y2H Array	32296183
Intra	UBE2D1	P51668	UBTD1	Homo sapiens	Q9HAC8	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	UBTD1	Homo sapiens	Q9HAC8	Y2H Array	32296183
Intra	UBE2D1	P51668	UBTD1	Homo sapiens	Q9HAC8	Validated Y2H	32296183
Intra	UBE2D1	P51668	RING1	Homo sapiens	Q06587	Y2H Array	19549727
Intra	UBE2D1	P51668	RING1	Homo sapiens	Q06587	Validated Y2H	32296183
Intra	UBE2D1	P51668	RING1	Homo sapiens	Q06587	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	RING1	Homo sapiens	Q06587	Y2H Array	32296183
Intra	UBE2D1	P51668	TRIM50	Homo sapiens	Q86XT4	Y2H Prey Pooling	32296183
Intra	UBE2D1	P51668	TRIM50	Homo sapiens	Q86XT4	Validated Y2H	32296183
Intra	UBE2D1	P51668	TRIM50	Homo sapiens	Q86XT4	Y2H Array	32296183
Cross	UBE2D1	P51668	RPS27A	Bos taurus	P62990	SLCA	21857666
Cross	UBE2D1	P51668	RPS27A	Bos taurus	P62990	Comigration in SDS	21857666
Cross	UBE2D1	P51668	Rnf4	Rattus norvegicus	O88846	Deubiquitinase Assay	21857666

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant UBE2D1 Proteins

Cat. No.	Product Name	Accession	Purity
HY-P71399	UBE2D1 Protein, Human (GST)	P51668 (M1-M147)	≥ 95%, as determined by reducing SDS-PAGE.

UBE2D1 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P83493	SFT Antibody (YA3238)	WB, IHC-P, FC, IP	Human, Mouse, Rat
HY-P83493A	SFT Antibody (YA3238)(PBS only)	WB, IHC-P, FC, IP	Human, Mouse, Rat

Related Diseases

Diseases	Alias
Tonne-Kalscheuer Syndrome	TOKAS Mrx61 Intellectual Developmental Disorder With Or Without Hand And Foot Anomalies, Genital Anomalies, Or Congenital Diaphragmatic Hernia Mental Retardation, X-Linked 61 X-Linked Mental Retardation 61
Long Qt Syndrome	Romano-Ward Syndrome Long Q-T Syndrome Lqt Qt Syndrome, Long Congenital Long Qt Syndrome Familial Long Qt Syndrome
Charcot-Marie-Tooth Disease	Cmt Hmsn Hereditary Motor And Sensory Neuropathy Pma Cmt - Charcot-Marie-Tooth Disease Charcot Marie Tooth Disease Charcot-Marie-Tooth Hereditary Neuropathy Charcot-Marie-Tooth Syndrome Peroneal Muscular Atrophy Hereditary Motor And Sensory Neuropathies

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (3)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS15339	UBE2D1 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS18719	Ube2d1 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS25206	Ube2d1 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Canis familiaris	UBE2D1	VGNC	VGNC:48050
Macaca mulatta	UBE2D1	VGNC	VGNC:78693
Felis catus	UBE2D1	VGNC	VGNC:66758
Rattus norvegicus	UBE2D1	RGD	RGD:1307886
Mus musculus	UBE2D1	MGD	MGI:2384911
Bos taurus	UBE2D1	VGNC	VGNC:36579
Others	UBE2D1	NCBI