14-3-3 eta Antibody (YA838)

Cat. No.: HY-P80525: FAQs
Data Sheet SDS COA User Guide for Antibodies

14-3-3 eta Antibody (YA838) is a non-conjugated and Mouse origined monoclonal antibody about 28 kDa, targeting to 14-3-3 eta (5F2). It can be used for WB assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μL	$70	In-stock	Estimated Time of Arrival: December 31
50 μL	$185	In-stock	Estimated Time of Arrival: December 31
100 μL	$300	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Biological Activity
Technical Parameters
Properties
Documentation

Description	14-3-3 eta Antibody (YA838) is a non-conjugated and Mouse origined monoclonal antibody about 28 kDa, targeting to 14-3-3 eta (5F2). It can be used for WB assays with tag free, in the background of Human, Mouse, Rat.
Background	14-3-3 eta (5F2): This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]
Tag	Free
Gene ID	7533
Synonyms	14-3-3 protein eta; 1433F_HUMAN; Brain protein 14-3-3; eta isoform; HGNC:12853; Protein AS1; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide; YWHA 1; YWHA1; Ywhah.
Molecular Weight	Predicted band size: 28 kDa;Observed band size: 28 kDa
Purity	affinity purified
Conjugation	Non-conjugated
Research Field	Neuroscience
Product Categories	Primary Antibody; Mouse Monoclonal Antibody
Host	Mouse
Reactivity	Human, Mouse, Rat
SwissProt ID	Q04917
Dilution Ratio	WB: 1:500-1:1,000 Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and Hela(lane 4(20μg) or lane 5(40μg) ) using 14-3-3 eta(HY-P80525) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80525, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Application	WB
Appearance	Liquid
Formulation	Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Immunogen	Synthetic peptide corresponding to Human 14-3-3η.The exact sequence is proprietary to MCE.
Documentation	Select Batch: Data Sheet (232 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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14-3-3 eta Antibody (YA838)

Biological Activity

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Documentation

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