SMARCA1 Antibody (YA3932)(PBS only)

Cat. No.: HY-P84235A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

SMARCA1 Antibody (YA3932) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SMARCA1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SMARCA1 Antibody (YA3932) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SMARCA1.

Host

Mouse

Molecular Weight

Predicted band size: 123 kDa;

Observed band size: 123 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P28370

Gene ID

6594 [NCBI]

Immunogen

Purified recombinant fragment of human SMARCA1 (AA: 933-1070) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：ATPase that possesses intrinsic ATP-dependent chromatin-remodeling activity (PubMed:14609955, PubMed:15310751, PubMed:15640247, PubMed:28801535). ATPase activity is substrate-dependent, and is increased when nucleosomes are the substrate, but is also catalytically active when DNA alone is the substrate (PubMed:14609955, PubMed:15310751, PubMed:15640247). Catalytic subunit of ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:14609955, PubMed:15310751, PubMed:15640247, PubMed:28801535). Within the ISWI chromatin-remodeling complexes, slides edge- and center-positioned histone octamers away from their original location on the DNA template (PubMed:28801535). Catalytic activity and histone octamer sliding propensity is regulated and determined by components of the ISWI chromatin-remodeling complexes (PubMed:28801535). The BAZ1A-, BAZ1B-, BAZ2A- and BAZ2B-containing ISWI chromatin-remodeling complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template (PubMed:28801535). The CECR2- and RSF1-containing ISWI chromatin-remodeling complexes do not have the ability to slide mononucleosomes to the center of a DNA template (PubMed:28801535). Within the NURF-1 and CERF-1 ISWI chromatin remodeling complexes, nucleosomes are the preferred substrate for its ATPase activity (PubMed:14609955, PubMed:15640247). Within the NURF-1 ISWI chromatin-remodeling complex, binds to the promoters of En1 and En2 to positively regulate their expression and promote brain development (PubMed:14609955). May promote neurite outgrowth (PubMed:14609955). May be involved in the development of luteal cells (PubMed:16740656). Facilitates nucleosome assembly during DNA replication, ensuring replication fork progression and genomic stability by preventing replication stress and nascent DNA gaps (PubMed:39413208); Catalytically inactive when either DNA or nucleosomes are the substrate and does not possess chromatin-remodeling activity (PubMed:15310751, PubMed:28801535). Acts as a negative regulator of chromatin remodelers by generating inactive complexes (PubMed:15310751)

Subcellular Localization：Nucleus; Chromosome

Expression：
Tissue_specificity:Expressed in lung, breast, kidney, ovary, skeletal muscle and brain; Mainly expressed in non-neuronal tissues such as lung, breast, kidney, and ovary

Isoforms & Post-Translational Modification：P28370 has 2 isomers: P28370-2: 121142 Da (predicted); P28370-1: 122605 Da (predicted).

Subunit：May form homodimers (PubMed:15310751). Component of the ACF-1 ISWI chromatin remodeling complex at least composed of SMARCA1 and BAZ1A, which regulates the spacing of histone octamers on the DNA template to facilitate access to DNA (PubMed:28801535). Within the complex interacts with BAZ1A; the interaction is direct (PubMed:28801535). Component of the WICH-1 ISWI chromatin remodeling complex at least composed of SMARCA1 and BAZ1B/WSTF (PubMed:28801535). Within the complex interacts with BAZ1B/WSTF (PubMed:28801535). Component of the NoRC-1 ISWI chromatin remodeling complex at least composed of SMARCA1 and BAZ2A/TIP5 (PubMed:28801535). Within the complex interacts with BAZ2A/TIP5 (PubMed:28801535). Component of the BRF-1 ISWI chromatin remodeling complex at least composed of SMARCA1 and BAZ2B (PubMed:28801535). Within the complex interacts with BAZ2B (PubMed:28801535). Component of the NURF-1 ISWI chromatin remodeling complex (also called the nucleosome-remodeling factor (NURF) complex) at least composed of SMARCA1, BPTF, RBBP4 and RBBP7 (PubMed:14609955, PubMed:15640247, PubMed:28801535). Within the complex interacts with BPTF (PubMed:14609955, PubMed:15310751, PubMed:28801535). Within the complex interacts with RBBP4 and RBBP7 (PubMed:14609955, PubMed:15310751). Component of the CERF-1 ISWI chromatin remodeling complex (also called the CECR2-containing-remodeling factor (CERF) complex) at least composed of CECR2 and SMARCA1 (PubMed:15640247, PubMed:28801535). LUZP1 is detected as part of the CERF-1 complex in embryonic stem cells where it is involved in complex stabilization but is not detected in the complex in the testis (By similarity). Component of the RSF-1 ISWI chromatin remodeling complex at least composed of SMARCA1 and RSF1 (PubMed:15640247, PubMed:28801535). Within the complex interacts with RSF1 (PubMed:28801535). Interacts with PRLR (PubMed:16740656). Interacts with ERCC6 (PubMed:26030138)

Synonyms

SWI; ISWI; SWI2; SNF2L; SNF2L1; SNF2LB; SNF2LT; hSNF2L; NURF140

Documentation

User Guide for Antibodies (1077 KB)