GLI2 Antibody
(Synonyms: THP, GLI2, Zinc finger protein GLI2, GLI family zinc finger protein 2, Tax helper protein)GLI2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to GLI2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 | 1:100-200 |
Product Details
GLI2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to GLI2.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 128 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 167 kDa
Purified recombinant protein of human GLI2
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
GLI2 is a Transcription factor that acts as a key effector of the smoothened signaling pathway, and which plays a role in skeleton development. Specifically binds to the minimal GLI-consensus sequence 5'-GACCACCCA-3' to activate or repress the expression of target genes (By similarity). Functions both as a transcriptional activator and repressor: the full-length GLI2 form (Transcription activator GLI2) acts as a transcription activator following smoothened activation, while the Transcription repressor GLI2R, which is generated in absence of smoothened, acts as a transcription repressor (By similarity). The transcriptional activator form constitutes the major form of GLI2 (By similarity)[1][2][3][4][5][6][7][8].
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Subcellular Localization
Nucleus; Cytoplasm; Cell projection, cilium
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Expression
Tissue_Specificity: Expressed in breast cancers (at protein level). Isoform 1 and isoform 4 are expressed in HTLV-1-infected T-cell lines (at protein level). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines. Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines. -
Isoforms & Post-Translational Modification
GLI2 has 5 isoforms, P10070-5: amino acid length is 1586, molecular weight is 167783 Da (predicted); P10070-1: amino acid length is 1258, molecular weight is 133241 Da (predicted); P10070-2: amino acid length is 1241, molecular weight is 131659 Da (predicted); P10070-3: amino acid length is 829, molecular weight is 88065 Da (predicted); P10070-4: amino acid length is 812, molecular weight is 86482 Da (predicted).
Acetylation at Lys-757 inhibits Hh target gene expression, probably by impeding entry into chromatin thus preventing promoter occupancy. -
Subunit
Interacts with SUFU; sequestering GLI2 in the cytoplasm.
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SwissProt ID
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Synonyms
THP, GLI2, Zinc finger protein GLI2, GLI family zinc finger protein 2, Tax helper protein
Documentation
[1]. Roessler E, et al. A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2. Hum Mol Genet. 2005 Aug 1;14(15):2181-8. [Content Brief]
[2]. Varjosalo M, et al. Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling. Cell. 2008 May 2;133(3):537-48. [Content Brief]
[3]. Maloverjan A, et al. Identification of a novel serine/threonine kinase ULK3 as a positive regulator of Hedgehog pathway. Exp Cell Res. 2010 Feb 15;316(4):627-37. [Content Brief]
[4]. Wen X, et al. Kinetics of hedgehog-dependent full-length Gli3 accumulation in primary cilia and subsequent degradation. Mol Cell Biol. 2010 Apr;30(8):1910-22. [Content Brief]
[5]. França MM, et al. Novel heterozygous nonsense GLI2 mutations in patients with hypopituitarism and ectopic posterior pituitary lobe without holoprosencephaly. J Clin Endocrinol Metab. 2010 Nov;95(11):E384-91. [Content Brief]
[6]. Cherry AL, et al. Structural basis of SUFU-GLI interaction in human Hedgehog signalling regulation. Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2563-79. [Content Brief]
[7]. Han B, et al. FOXC1 Activates Smoothened-Independent Hedgehog Signaling in Basal-like Breast Cancer. Cell Rep. 2015 Nov 3;13(5):1046-58. [Content Brief]
[8]. Tanimura A, et al. Cloning of novel isoforms of the human Gli2 oncogene and their activities to enhance tax-dependent transcription of the human T-cell leukemia virus type 1 genome. J Virol. 1998 May;72(5):3958-64. [Content Brief]
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