DBF4 Antibody (YA2652)(PBS only)

Cat. No.: HY-P82907A: Data Sheet User Guide for Antibodies
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DBF4 Antibody (YA2652) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DBF4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DBF4 Antibody (YA2652) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DBF4.

Host

Rabbit

Molecular Weight

Predicted band size: 77 kDa;

Observed band size: 77 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9UBU7

Gene ID

10926 [NCBI]

Immunogen

A synthetic peptide of human DBF4

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:5000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Highly expressed in testis and thymus. Expressed also in most cancer cells lines

Induction:Induced in G1 phase at low level, increased during G1-S phase and remain high during S and G2-M phase

Isoforms & Post-Translational Modification：Q9UBU7 has 2 isomers: Q9UBU7-1: 76858 Da (predicted); Q9UBU7-2: 26124 Da (predicted).
Phosphorylation increases its interaction with PSIP1

Subunit：Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6. Interacts (via IBM motifs) with PSIP1 (via IBD domain); phosphorylation increases its affinity for PSIP1 (PubMed:29997176)

Research Field

Cell Biology

Synonyms

DBF4; ASK; DBF4A; ZDBF1; Protein DBF4 homolog A; Activator of S phase kinase; Chiffon homolog A; DBF4-type zinc finger-containing protein 1

Documentation

User Guide for Antibodies (1077 KB)