DBF4 Antibody (YA2652)(PBS only)
(Synonyms: DBF4; ASK; DBF4A; ZDBF1; Protein DBF4 homolog A; Activator of S phase kinase; Chiffon homolog A; DBF4-type zinc finger-containing protein 1)DBF4 Antibody (YA2652) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DBF4.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:2000-1:5000 |
Product Details
DBF4 Antibody (YA2652) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DBF4.
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Host Rabbit
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 77 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 77 kDa
A synthetic peptide of human DBF4
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
DBF4 is a Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:Highly expressed in testis and thymus. Expressed also in most cancer cells lines
Induction:Induced in G1 phase at low level, increased during G1-S phase and remain high during S and G2-M phase -
Isoforms & Post-Translational Modification
Q9UBU7 has 2 isomers: Q9UBU7-1: 76858 Da (predicted); Q9UBU7-2: 26124 Da (predicted).
Phosphorylation increases its interaction with PSIP1 -
Subunit
Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6. Interacts (via IBM motifs) with PSIP1 (via IBD domain); phosphorylation increases its affinity for PSIP1 (PubMed:29997176)
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SwissProt ID
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Synonyms
DBF4; ASK; DBF4A; ZDBF1; Protein DBF4 homolog A; Activator of S phase kinase; Chiffon homolog A; DBF4-type zinc finger-containing protein 1
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Research Field
Cell Biology
Documentation
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