Wnt3a Antibody (YA7354)

Cat. No.: HY-P87669: Data Sheet COA
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User Guide for Antibodies
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Wnt3a Antibody (YA7354) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Wnt3a.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Wnt3a Antibody (YA7354) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Wnt3a.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 39 kDa;

Observed band size: 39 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P56704

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200

Sensitivity	Endogenous	Purity	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG,Kappa

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:20093360, PubMed:21244856, PubMed:24841207, PubMed:26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed:26902720)

Subcellular Localization：Secreted, extracellular space, extracellular matrix,Secreted

Expression：
Tissue_Specificity: Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate

Isoforms & Post-Translational Modification：P56704 has two isomers: P56704-1: 39365 Da (predicted); P56704-2: 42905 Da (predicted).
Palmitoleoylation by PORCN is required for efficient binding to frizzled receptors丨Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT3A丨Disulfide bonds have critical and distinct roles in secretion and activity

Subunit：Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720)

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)