Phospho-ELK1 (Ser383) Antibody (YA9880)
(Synonyms: ETS domain-containing protein Elk-1)Based on 1 Customer Validation
Phospho-ELK1 (Ser383) Antibody (YA9880) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-ELK1 (Ser383).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IP
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Reactivity :
Human, Mouse
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Formulation:
Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:5000 | 1:1000 | Recomend 1-2μg per 100-500μg Total protein |
Product Details
Phospho-ELK1 (Ser383) Antibody (YA9880) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-ELK1 (Ser383).
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Host Rabbit
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 45 kDa
Synthetic phosphopeptide corresponding to residues surrounding Ser383 of human ELK1.
Endogenous
affinity purified
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from RAW264.7 (lane 2, 20ug), RAW264.7 treated with 5μg/mL LPS for 8 hours (lane3, 20μg) and RAW264.7 treated with 10μg/mL LPS for 8 hours (lane4, 20μg) using Phospho-ELK1 (Ser383) Antibody (HY-P810618). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
Background
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Function
ELK1 is a Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK and MAPK-signaling pathways stimulation[1][2][3].
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Subcellular Localization
Nucleus
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Expression
Tissue_Specificity: Lung and testis. -
Isoforms & Post-Translational Modification
Phospho-ELK1 has 2 isoforms, P19419-1: amino acid length is 428, molecular weight is 44888 Da (predicted); P19419-2: amino acid length is 95, molecular weight is 11217 Da (predicted).Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention
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Subunit
Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity.
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SwissProt ID
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Synonyms
ETS domain-containing protein Elk-1
Documentation
References
[1]. Huang Y, et al. Characterization of hPRP4 kinase activation: potential role in signaling. Biochem Biophys Res Commun. 2000 May 10;271(2):456-63. [Content Brief]
[2]. Gille H, et al. ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation. EMBO J. 1995 Mar 1;14(5):951-62. [Content Brief]
[3]. Janknecht R, et al. Elk-1 protein domains required for direct and SRF-assisted DNA-binding. Nucleic Acids Res. 1992 Jul 11;20(13):3317-24. [Content Brief]
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