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KMT5A Antibody (YA1376)

(Synonyms: KMT5A; PR-Set7; SET07; SET8)
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Based on 1 Customer Validation

KMT5A Antibody (YA1376) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KMT5A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KMT5A Antibody (YA1376)
  • KMT5A Antibody (YA1376) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:50-1:100

Product Details

Description

KMT5A Antibody (YA1376) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KMT5A.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human KMT5A / SETD8 / Pr-SET7 aa50-105/393.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KMT5A Antibody (YA1376)
    Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), HCT116 (lane 3), SH-SY5Y (lane 4),U87 (lane 5, Negative), HeLa (lane 6), and Mouse brain (lane 7) using KMT5A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    KMT5A is a Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Nucleus; Chromosome

  • Expression


    Induction:By HCFC1 C-terminal chain, independently of HCFC1 N-terminal chain. Transiently induced by TGF-beta and during the cell cycle

  • Isoforms & Post-Translational Modification

    Q9NQR1 has 2 isomers: Q9NQR1-1: 42890 Da (predicted); Q9NQR1-2: 39223 Da (predicted).
    Acetylated at Lys-162; does not affect methyltransferase activity. Deacetylated at Lys-162 possibly by SIRT2; does not change methyltransferase activity;Ubiquitinated and degraded by the DCX(DTL) complex

  • Subunit

    Interacts with L3MBTL1

  • SwissProt ID

    Q9NQR1

  • Gene ID
    387893 [NCBI]
  • Synonyms

    KMT5A; PR-Set7; SET07; SET8

  • Research Field

    Epigenetics and Nuclear Signaling

KMT5A Antibody (YA1376) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), HCT116 (lane 3), SH-SY5Y (lane 4),U87 (lane 5, Negative), HeLa (lane 6), and Mouse brain (lane 7) using KMT5A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • KMT5A Antibody (YA1376) Product Image
    1/16
  • Experimental Validation Results for KMT5A Antibody (YA1376)
  • KMT5A Antibody (YA1376) Product Image
  • Experimental Validation Results for KMT5A Antibody (YA1376)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), HCT116 (lane 3), SH-SY5Y (lane 4),U87 (lane 5, Negative), HeLa (lane 6), and Mouse brain (lane 7) using KMT5A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for KMT5A Antibody (YA1376)
  • KMT5A Antibody (YA1376) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), HCT116 (lane 3), SH-SY5Y (lane 4),U87 (lane 5, Negative), HeLa (lane 6), and Mouse brain (lane 7) using KMT5A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • KMT5A Antibody (YA1376) Product Image WB