MST1 Antibody (YA2368)

(Synonyms: HGFL; MSP; MST1; NF15S2)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

MST1 Antibody (YA2368) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MST1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated

Publications Citing Use of MedChemExpress (MCE) MST1 Antibody (YA2368)

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

MST1 Antibody (YA2368) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MST1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 96 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 80 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human MST1.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104723

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MST1 Antibody (YA2368)
    Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), C6 (lane 2, 20 μg), COS-7 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), and HepG2 (lane 5, 20 μg) using MST1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for MST1 Antibody (YA2368)
    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue using MST1 Antibody (HY-P82623, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MST1 Antibody (YA2368)
    Immunohistochemical analysis of paraffin-embedded human glioma tissue using MST1 Antibody (HY-P82623, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MST1 Antibody (YA2368)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using MST1 Antibody (HY-P82623, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MST1 Antibody (YA2368)
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using MST1 Antibody (HY-P82623, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MST1 Antibody (YA2368)
    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using MST1 Antibody (HY-P82623, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MST1 Antibody (YA2368)
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using MST1 Antibody (HY-P82623, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MST1 Antibody (YA2368)
    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using MST1 Antibody (HY-P82623, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MST1 Antibody (YA2368)
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using MST1 Antibody (HY-P82623, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    MST1/STK4 protein is a stress-activated, pro-apoptotic kinase with diverse cellular functions, including a critical role in the Hippo signaling pathway. Following caspase-cleavage, MST1/STK4 enters the nucleus, inducing chromatin condensation and internucleosomal DNA fragmentation, contributing to apoptosis. In the Hippo pathway, MST1/STK4, along with its regulatory protein SAV1, forms a kinase cascade that phosphorylates and activates LATS1/2-MOB1 complex. This, in turn, phosphorylates and inactivates the YAP1 oncoprotein and WWTR1/TAZ, regulating genes involved in proliferation, cell death, and cell migration. MST1/STK4 is essential for repressing hepatocyte proliferation, inhibiting facultative adult liver stem cells, and preventing tumor formation. Additionally, it plays a role in histone modification during apoptosis, phosphorylates various substrates like FOXO3, MOBKL1A, MOBKL1B, RASSF2, TNNI3, and FOXO1, influencing diverse cellular processes such as transcription, cell death initiation, and signaling pathways like PKB/AKT1 and AR. Notably, MST1/STK4 acts as an inhibitor of PKB/AKT1, and its phosphorylation of SIRT1 promotes p53-dependent transcription and apoptosis in response to DNA damage. Furthermore, it intersects with PKB/AKT1 signaling to suppress AR activity by antagonizing the formation of AR-chromatin complexes.

  • Subcellular Localization

    Secreted

  • Subunit

    Dimer of an alpha chain and a beta chain linked by a disulfide bond. Interacts (via beta chain) with MST1R (via SEMA domain)

  • SwissProt ID

    P26927

  • Gene ID
  • Synonyms

    HGFL; MSP; MST1; NF15S2

  • Research Field

    Cell Biology

MST1 Antibody (YA2368) Related Classifications

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100 mg

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