1. Stem Cell/Wnt
  2. Hippo (MST)

XMU-MP-1 

Cat. No.: HY-100526 Purity: 99.34%
Handling Instructions

XMU-MP-1 is a reversible and selective MST1/2 inhibitor with IC50 values of 71.1 and 38.1 nM against MST1 and MST2.

For research use only. We do not sell to patients.

XMU-MP-1 Chemical Structure

XMU-MP-1 Chemical Structure

CAS No. : 2061980-01-4

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 119 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
2 mg USD 90 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
5 mg USD 130 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
10 mg USD 190 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
25 mg USD 390 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
50 mg USD 650 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
100 mg USD 990 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

    XMU-MP-1 purchased from MCE. Usage Cited in: Cell Rep. 2017 Dec 19;21(12):3612-3623.

    Control and SLMAP mutant HAP1 cells are probed for phosphorylation of MST1/2 (T183/180), YAP (S127 and S397) and MOB1 (T35). Note increased phosphorylation of these proteins in the SLMAP mutant cells, which is completely suppressed by XMU-MP-1.

    XMU-MP-1 purchased from MCE. Usage Cited in: Nat Immunol. 2017 Sep;18(9):973-984.

    Spp1 expression in BMMs cultured (1×105 per well) in flat- or round-bottomed plates (horizontal axis) with (XMU-MP-1) or without (Vehicle) an inhibitor of Mst1 and Mst2 (5 μg/mL); results are presented relative to those of vehicle-treated cells cultured on flat-bottomed plates.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    XMU-MP-1 is a reversible and selective MST1/2 inhibitor with IC50 values of 71.1 and 38.1 nM against MST1 and MST2.

    IC50 & Target

    IC50: 71.1 (MST1), 38.1 nM (MST2)[1]

    In Vitro

    At concentrations ranging from 0.1 to 10 μM, XMU-MP-1 reduces the phosphorylation of endogenous MOB1, LATS1/2, and YAP in HepG2 cells in a dose-dependent manner. XMU-MP-1 treatment inhibits hydrogen peroxide-stimulated MOB1 phosphorylation and MST1/2 autophosphorylation in a variety of cell lines, including mouse macrophage-like cells, human osteosarcoma, human colorectal adenocarcinoma cells. XMU-MP-1 blocks MST1/2 kinase activities, thereby activating the downstream effector Yes-associated protein and promoting cell growth. XMU-MP-1 can potently and reversibly suppress the activities of kinases MST1/2 and enhance their downstream YAP activation in cells[1].

    In Vivo

    XMU-MP-1 displays excellent in in vivo pharmacokinetics and is able to augment mouse intestinal repair, as well as liver repair and regeneration, in both acute and chronic liver injury mouse models at a dose of 1 to 3 mg/kg via intraperitoneal injection. XMUMP-1 treatment exhibits substantially greater repopulation rate of human hepatocytes in the Fah-deficient mouse model than in the vehicle-treated control, indicating that XMU-MP-1 treatment might facilitate human liver regeneration[1].

    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 2.4011 mL 12.0054 mL 24.0108 mL
    5 mM 0.4802 mL 2.4011 mL 4.8022 mL
    10 mM 0.2401 mL 1.2005 mL 2.4011 mL
    Please refer to the solubility information to select the appropriate solvent.
    Kinase Assay
    [1]

    XMU-MP-1 is dissolved in DMSO (stock concentration, 10 mM). For the in vitro kinase inhibition assays, recombinant GST-tagged MOB1a and various forms of recombinant His-tagged full-length MST1 or MST2 kinase are expressed and purified from Escherichia coli. The assays are performed with the various doses of XMU-MP-1 in the kinase assay buffer for 30 min at 30°C[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    416.48

    Formula

    C₁₇H₁₆N₆O₃S₂

    CAS No.

    2061980-01-4

    SMILES

    O=S(C1=CC=C(NC2=NC=C(C(N(C)C3=C4SC=C3)=N2)N(C)C4=O)C=C1)(N)=O

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 30 mg/mL

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    Purity: 99.34%

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product name

     

    Salutation

    Applicant name *

     

    Email address *

    Phone number *

     

    Organization name *

    Country or Region *

     

    Requested quantity *

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    XMU-MP-1
    Cat. No.:
    HY-100526
    Quantity: