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  4. Monoclonal Antibodies Recombinant Antibodies
  5. Nesprin3 Antibody (YA1371)

Nesprin3 Antibody (YA1371)

Cat. No.: HY-P81626
User Guide for Antibodies Technical Support

Nesprin3 Antibody (YA1371) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nesprin3.

For research use only. We do not sell to patients.

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50 μL In-stock
100 μL In-stock
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

Nesprin3 Antibody (YA1371) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nesprin3.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 112 kDa;
Observed band size: 112 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Recombinant protein of human Nesprin3
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:5000
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells
Subcellular Localization：Nucleus outer membrane; Single-pass type IV membrane protein; Nucleus envelope; Rough endoplasmic reticulum
Expression：
Tissue_specificity:Expressed in aortic endothelial cells (at protein level)
Isoforms & Post-Translational Modification：Q6ZMZ3 has 3 isomers: Q6ZMZ3-1: 112216 Da (predicted); Q6ZMZ3-2: 111677 Da (predicted); Q6ZMZ3-3: 68582 Da (predicted).
The disulfid bond with SUN1 or SUN2 is required for stability of the respective LINC complex under tensile forces
Subunit：Core component of LINC complexes which are composed of inner nuclear membrane SUN domain-containing proteins coupled to outer nuclear membrane KASH domain-containing nesprins. SUN and KASH domain-containing proteins seem to bind each other promiscuously; however, differentially expression of LINC complex constituents can give rise to specific assemblies. Interacts with SUN1 and SUN2; probably forming respective LINC complexes. Interacts with PLEC (via actin-binding domain). Interacts with DST. Interacts with SYNE1 via spectrin repeats. Interacts (via KASH domain) with TOR1A (ATP-bound); the interaction is required for SYNE3 nuclear envelope localization
Database

Entrez Gene: 161176 Human

SwissProt: Q6ZMZ3 Human

OMIM: 610861 Human

Research Field

Signal Transduction
Synonyms
KASH3; NET53; Nesp3; C14orf49; C14orf139; LINC00341; NCRNA00341
Documentation
User Guide for Antibodies (1077 KB)

Nesprin3 Antibody (YA1371) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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