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COX2 Antibody (YA493)

(Synonyms: PTGS2; COX 2; COX2; COX-2; Cyclooxygenase; Cyclooxygenase2; Cyclooxygenase-2; hCox 2; PGG/HS; PGH synthase 2; PGHS 2; PGHS2; PHS 2; PHS II; PHS2 ; Prostaglandin endoperoxide synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; TIS10II; PGH2_HUMAN. )
3 Cited Publications
Customer Review

Based on 3 publication(s) in Google Scholar

COX2 Antibody (YA493) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to COX2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P
  • Experimental Validation Results for COX2 Antibody (YA493)
  • Experimental Validation Results for COX2 Antibody (YA493)
  • Experimental Validation Results for COX2 Antibody (YA493)
  • Experimental Validation Results for COX2 Antibody (YA493)
  • Experimental Validation Results for COX2 Antibody (YA493)
  • COX2 Antibody (YA493) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:5000 1:50-1:200 1:100

Product Details

Description

COX2 Antibody (YA493) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to COX2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 69 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 69 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human COX2.AA range:100-140.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102298

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for COX2 Antibody (YA493)
    Western blot analysis of extracts from C2C12 (lane 2(20μg), C2C12 (lane 3(20μg),using COX2 Antibody (HY-P80091) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for COX2 Antibody (YA493)
    Immunocytochemistry analysis of A549 cells labeling COX2 with COX2 Antibody (HY-P80091) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with COX2 Antibody (HY-P80091) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.
  • Experimental Validation Results for COX2 Antibody (YA493)
    Immunocytochemistry analysis of C2C12 cells labeling COX2 with COX2 Antibody (HY-P80091) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with COX2 Antibody (HY-P80091)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.
  • Experimental Validation Results for COX2 Antibody (YA493)
    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using COX2/Cyclooxygenase 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80091, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for COX2 Antibody (YA493)
    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using COX2/Cyclooxygenase 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80091, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Cyclooxygenase-2 (COX-2) is an inducible enzyme catalyzing the conversion of arachidonic acid to prostaglandin endoperoxides, central to prostanoid biosynthesis and inflammatory signaling[1][2]. Mechanistically, COX-2 activity is regulated by both its catalytic and allosteric subunits, allowing modulation by fatty acids and selective inhibitors, distinguishing it from constitutive COX-1[2]. COX-2 is strongly upregulated in response to pro-inflammatory cytokines, hypoxia, and tumor microenvironment signals, orchestrating prostaglandin E2 (PGE2) production and downstream activation of NF-κB and PI3K-Akt pathways[3][4][5]. In disease models, COX-2 contributes to cancer progression, atherosclerosis, and migraine through modulation of immune responses, vascular smooth muscle activity, and neuronal-glial interactions[6][7][5]. Compared with COX-1, COX-2 expression is more context-dependent and spatially regulated, being highly inducible in endothelial cells, arterial versus venous smooth muscle, and neuronal populations under stress or inflammatory stimuli[8][9][10]. Experimental studies demonstrate COX-2 as a critical mediator of ischemic preconditioning in the heart, providing cardioprotection via PGE2 and PGI2 signaling[11]. Selective COX-2 inhibitors, including synthetic NSAIDs and dual COX/LOX modulators, are utilized to interrogate COX-2 function and develop anti-inflammatory therapies with reduced gastrointestinal and cardiovascular side effects[3][12]. Agonist studies further highlight receptor-mediated induction of COX-2, exemplifying its role in colon carcinoma and trigeminal ganglia models[13][7].

  • Subcellular Localization

    Microsome membrane; Peripheral membrane protein; Endoplasmic reticulum membrane; Peripheral membrane protein; Nucleus inner membrane; Peripheral membrane protein; Nucleus outer membrane; Peripheral membrane protein

  • Expression


    Induction:By cytokines and mitogens. Up-regulated by IL1B (PubMed:26282205, PubMed:9545330) . Up-regulated by lipopolysaccharide (LPS) (PubMed:9545330)

  • Subunit

    Homodimer

  • SwissProt ID

    P35354

  • Gene ID
    5743 [NCBI]
  • Synonyms

    PTGS2; COX 2; COX2; COX-2; Cyclooxygenase; Cyclooxygenase2; Cyclooxygenase-2; hCox 2; PGG/HS; PGH synthase 2; PGHS 2; PGHS2; PHS 2; PHS II; PHS2 ; Prostaglandin endoperoxide synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; TIS10II; PGH2_HUMAN.

  • Research Field

    Immunology

[1]. Smith FG, et al. Cyclooxygenase (COX) Inhibitors and the Newborn Kidney. Pharmaceuticals (Basel). 2012 Oct 25;5(11):1160-76. [Content Brief]

[2]. Ahmadi M, et al. Non-steroidal anti-inflammatory drugs: recent advances in the use of synthetic COX-2 inhibitors. RSC Med Chem. 2022 Feb 14;13(5):471-496. [Content Brief]

[3]. Bolli R, et al. Discovery of a new function of cyclooxygenase (COX)-2: COX-2 is a cardioprotective protein that alleviates ischemia/reperfusion injury and mediates the late phase of preconditioning. Cardiovasc Res. 2002 Aug 15;55(3):506-19. [Content Brief]

[4]. Kirkby NS, et al. COX-2 protects against atherosclerosis independently of local vascular prostacyclin: identification of COX-2 associated pathways implicate Rgl1 and lymphocyte networks. PLoS One. 2014 Jun 2;9(6):e98165. [Content Brief]

[5]. Segelcke D, et al. The role of the spinal cyclooxygenase (COX) for incisional pain in rats at different developmental stages. Eur J Pain. 2020 Feb;24(2):312-324. [Content Brief]

[6]. Chatzipieris FP, et al. Recent advances in dual COX/LOX inhibitor design (2020-2024). Life. 2026;16(1):163. [Content Brief]

[7]. Yang WL, et al. Cholinergic receptor up-regulates COX-2 expression and prostaglandin E(2) production in colon cancer cells. Carcinogenesis. 2000 Oct;21(10):1789-93. [Content Brief]

[8]. Hsieh HL, et al. c-Src-dependent EGF receptor transactivation contributes to ET-1-induced COX-2 expression in brain microvascular endothelial cells. J Neuroinflammation. 2012 Jul 2;9:152. [Content Brief]

[9]. Neeb L, et al. IL-1β stimulates COX-2 dependent PGE₂ synthesis and CGRP release in rat trigeminal ganglia cells. PLoS One. 2011 Mar 4;6(3):e17360. [Content Brief]

[10]. Chen S, et al. 288 The COX-2 pathway as a mediator of resistance to anti-PD-1 therapy. J Immunother Cancer. 2021;9(Suppl 2):A312.

[11]. Bishop-Bailey D, et al. Differential induction of cyclooxygenase-2 in human arterial and venous smooth muscle: role of endogenous prostanoids. Arterioscler Thromb Vasc Biol. 1998 Oct;18(10):1655-61. [Content Brief]

[12]. Majumder M, et al. COX-2 Elevates Oncogenic miR-526b in Breast Cancer by EP4 Activation. Mol Cancer Res. 2015 Jun;13(6):1022-33. [Content Brief]

[13]. Scott KF, et al. Functional coupling and differential regulation of the phospholipase A2-cyclooxygenase pathways in inflammation. J Leukoc Biol. 1999 Oct;66(4):535-41. [Content Brief]

[14]. Smith WL, et al. Interactions of fatty acids, nonsteroidal anti-inflammatory drugs, and coxibs with the catalytic and allosteric subunits of cyclooxygenases-1 and -2. J Biol Chem. 2019 Feb 1;294(5):1697-1705. [Content Brief]

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COX2 Antibody (YA493) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C2C12 (lane 2(20μg), C2C12 (lane 3(20μg),using COX2 Antibody (HY-P80091) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling COX2 with COX2 Antibody (HY-P80091) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with COX2 Antibody (HY-P80091) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of C2C12 cells labeling COX2 with COX2 Antibody (HY-P80091) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with COX2 Antibody (HY-P80091)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using COX2/Cyclooxygenase 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80091, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using COX2/Cyclooxygenase 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80091, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • COX2 Antibody (YA493) Product Image
    1/16
  • Experimental Validation Results for COX2 Antibody (YA493)
  • Experimental Validation Results for COX2 Antibody (YA493)
  • Experimental Validation Results for COX2 Antibody (YA493)
  • Experimental Validation Results for COX2 Antibody (YA493)
  • Experimental Validation Results for COX2 Antibody (YA493)
  • COX2 Antibody (YA493) Product Image
  • Experimental Validation Results for COX2 Antibody (YA493)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C2C12 (lane 2(20μg), C2C12 (lane 3(20μg),using COX2 Antibody (HY-P80091) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for COX2 Antibody (YA493)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling COX2 with COX2 Antibody (HY-P80091) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with COX2 Antibody (HY-P80091) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.
  • Experimental Validation Results for COX2 Antibody (YA493)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of C2C12 cells labeling COX2 with COX2 Antibody (HY-P80091) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with COX2 Antibody (HY-P80091)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.
  • Experimental Validation Results for COX2 Antibody (YA493)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using COX2/Cyclooxygenase 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80091, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for COX2 Antibody (YA493)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using COX2/Cyclooxygenase 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80091, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for COX2 Antibody (YA493)
  • Experimental Validation Results for COX2 Antibody (YA493)
  • Experimental Validation Results for COX2 Antibody (YA493)
  • Experimental Validation Results for COX2 Antibody (YA493)
  • Experimental Validation Results for COX2 Antibody (YA493)
  • COX2 Antibody (YA493) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C2C12 (lane 2(20μg), C2C12 (lane 3(20μg),using COX2 Antibody (HY-P80091) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • COX2 Antibody (YA493) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling COX2 with COX2 Antibody (HY-P80091) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with COX2 Antibody (HY-P80091) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

  • COX2 Antibody (YA493) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of C2C12 cells labeling COX2 with COX2 Antibody (HY-P80091) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with COX2 Antibody (HY-P80091)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.

  • COX2 Antibody (YA493) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using COX2/Cyclooxygenase 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80091, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • COX2 Antibody (YA493) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using COX2/Cyclooxygenase 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80091, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • COX2 Antibody (YA493) Product Image WB
  • COX2 Antibody (YA493) Product Image ICC
  • COX2 Antibody (YA493) Product Image ICC
  • COX2 Antibody (YA493) Product Image IHC-P
  • COX2 Antibody (YA493) Product Image IHC-P