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Pan-Cadherin Antibody (YA247)

(Synonyms: Cadherin antibody; CDH3 antibody; CDHP antibody; 7B4 antigen antibody; Cadherin-1 antibody; Cadherin-2 antibody; Cadherin-3 antibody; Cadherin-4 antibody; Cadherin-5 antibody; CAM 120/80 antibody; CD144 antibody; CD324 antibody; CD325 antibody; Cdh4 antibody; CDH5 antibody; CDHE antibody; CDHN antibody; E-cadherin antibody; Epithelial cadherin antibody; N-cadherin antibody; NCAD antibody; Neural cadherin antibody; P-cadherin antibody; Placental cadherin antibody; UVO antibody; Uvomorulin antibody)
Customer Review

Based on 1 Customer Validation

Pan-Cadherin Antibody (YA247) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pan-Cadherin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC ICC
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Pan-Cadherin Antibody (YA247) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000 1:50-1:200 1:50-1:1000 1:50-1:100 Use at an assay dependent concentration.

Product Details

Description

Pan-Cadherin Antibody (YA247) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pan-Cadherin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 130 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 100 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within C-terminal human CDH2.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102871

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
    Western blot analysis of extracts from HEK293T (lane2(20μg), C2C12 (lane3(20μg), C6 (lane4(20μg) using Pan-Cadherin Antibody (HY-P80262). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Pan-Cadherin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80262, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Pan-Cadherin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80262, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
    Flow cytometric analysis of 1X106 C2C12 cells labeling Pan-Cadherin Antibody (HY-P80262, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
    Immunocytochemistry analysis of C6 cells labeling Pan-Cadherin with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
    Immunocytochemistry analysis of Hela cells labeling Pan-Cadherin with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilutionCells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Pan-Cadherin Antibody (HY-P80262)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Pan-Cadherin is a Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells. Required for proper neurite branching. Required for pre- and postsynaptic organization. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell membrane, sarcolemma; Cell junction; Cell surface; Cell junction, desmosome; Cell junction, adherens junction

  • Subunit

    Homodimer (via extracellular region). Can also form heterodimers with other cadherins (via extracellular region). Dimerization occurs in trans, i.e. with a cadherin chain from another cell (By similarity). Interacts with CDCP1 (PubMed:16007225). Interacts with PCDH8; this complex may also include TAOK2 (By similarity). The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway (By similarity). Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. May interact with OBSCN (via protein kinase domain 2) (By similarity). Interacts with FBXO45 (PubMed:32341084)

  • SwissProt ID

    P19022

  • Gene ID
    1001 [NCBI]
  • Synonyms

    Cadherin antibody; CDH3 antibody; CDHP antibody; 7B4 antigen antibody; Cadherin-1 antibody; Cadherin-2 antibody; Cadherin-3 antibody; Cadherin-4 antibody; Cadherin-5 antibody; CAM 120/80 antibody; CD144 antibody; CD324 antibody; CD325 antibody; Cdh4 antibody; CDH5 antibody; CDHE antibody; CDHN antibody; E-cadherin antibody; Epithelial cadherin antibody; N-cadherin antibody; NCAD antibody; Neural cadherin antibody; P-cadherin antibody; Placental cadherin antibody; UVO antibody; Uvomorulin antibody

  • Research Field

    Tags & Cell Markers

Pan-Cadherin Antibody (YA247) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane2(20μg), C2C12 (lane3(20μg), C6 (lane4(20μg) using Pan-Cadherin Antibody (HY-P80262). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Pan-Cadherin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80262, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Pan-Cadherin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80262, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 C2C12 cells labeling Pan-Cadherin Antibody (HY-P80262, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Pan-Cadherin with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Pan-Cadherin with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilutionCells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Pan-Cadherin Antibody (HY-P80262)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Pan-Cadherin Antibody (YA247) Product Image
    1/16
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Pan-Cadherin Antibody (YA247) Product Image
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane2(20μg), C2C12 (lane3(20μg), C6 (lane4(20μg) using Pan-Cadherin Antibody (HY-P80262). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Pan-Cadherin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80262, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Pan-Cadherin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80262, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 C2C12 cells labeling Pan-Cadherin Antibody (HY-P80262, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Pan-Cadherin with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Pan-Cadherin with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilutionCells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Pan-Cadherin Antibody (HY-P80262)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Experimental Validation Results for Pan-Cadherin Antibody (YA247)
  • Pan-Cadherin Antibody (YA247) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T (lane2(20μg), C2C12 (lane3(20μg), C6 (lane4(20μg) using Pan-Cadherin Antibody (HY-P80262). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Pan-Cadherin Antibody (YA247) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Pan-Cadherin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80262, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Pan-Cadherin Antibody (YA247) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Pan-Cadherin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80262, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Pan-Cadherin Antibody (YA247) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 C2C12 cells labeling Pan-Cadherin Antibody (HY-P80262, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Pan-Cadherin Antibody (YA247) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of C6 cells labeling Pan-Cadherin with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Pan-Cadherin Antibody (YA247) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Pan-Cadherin with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilutionCells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Pan-Cadherin Antibody (HY-P80262)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Pan-Cadherin Antibody (YA247) Product Image WB
  • Pan-Cadherin Antibody (YA247) Product Image IHC-P
  • Pan-Cadherin Antibody (YA247) Product Image IHC-P
  • Pan-Cadherin Antibody (YA247) Product Image FC
  • Pan-Cadherin Antibody (YA247) Product Image ICC
  • Pan-Cadherin Antibody (YA247) Product Image ICC