Pan-Cadherin Antibody (YA247)

Cat. No.: HY-P80262: Data Sheet COA
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Pan-Cadherin Antibody (YA247) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pan-Cadherin.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Pan-Cadherin Antibody (YA247) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Pan-Cadherin Antibody (YA247) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pan-Cadherin.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 100 kDa;

Observed band size: 130 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P19022

Gene ID

1001 [NCBI]

Immunogen

Synthetic peptide within C-terminal human CDH2.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:1000
FC FC: Flow Cytometry	1:50-1:100
IP IP: Immunoprecipitation	Use at an assay dependent concentration.

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL ICC IHC-P

Immunocytochemistry analysis of C6 cells labeling Pan-Cadherin with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of Hela cells labeling Pan-Cadherin with Pan-Cadherin Antibody (HY-P80262) at 1/50 dilutionCells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Pan-Cadherin Antibody (HY-P80262)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Pan-Cadherin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80262, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Pan-Cadherin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80262, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell membrane, sarcolemma; Cell junction; Cell surface; Cell junction, desmosome; Cell junction, adherens junction

Subunit：Homodimer (via extracellular region). Can also form heterodimers with other cadherins (via extracellular region). Dimerization occurs in trans, i.e. with a cadherin chain from another cell (By similarity). Interacts with CDCP1 (PubMed:16007225). Interacts with PCDH8; this complex may also include TAOK2 (By similarity). The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway (By similarity). Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. May interact with OBSCN (via protein kinase domain 2) (By similarity). Interacts with FBXO45 (PubMed:32341084)

RRID

AB_3102871

Database

Entrez Gene: 1001 Human ; 1002 Human ; 1003 Human ; 1004 Human ; 1005 Human ; 1006 Human ; 1008 Human ; 1009 Human ; 1012 Human ; 1014 Human ; 1015 Human ; 999 Human ;

SwissProt: P12830 Human ; P19022 Human ; P22223 Human ; P33151 Human ; P55283 Human ; P39038 Mouse ;

OMIM: 137215 Human

Research Field

Tags & Cell Markers

Synonyms

Cadherin antibody; CDH3 antibody; CDHP antibody; 7B4 antigen antibody; Cadherin-1 antibody; Cadherin-2 antibody; Cadherin-3 antibody; Cadherin-4 antibody; Cadherin-5 antibody; CAM 120/80 antibody; CD144 antibody; CD324 antibody; CD325 antibody; Cdh4 antibody; CDH5 antibody; CDHE antibody; CDHN antibody; E-cadherin antibody; Epithelial cadherin antibody; N-cadherin antibody; NCAD antibody; Neural cadherin antibody; P-cadherin antibody; Placental cadherin antibody; UVO antibody; Uvomorulin antibody

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Pan-Cadherin Antibody (YA247) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Pan-Cadherin Antibody (YA247)

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Background

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Pan-Cadherin Antibody (YA247) Related Classifications

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