CD205 Antibody (YA9977)
(Synonyms: CD205, Cd205, SMU_610, spaP, Lymphocyte antigen 75, Ly-75, DEC-205)CD205 Antibody (YA9977) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to CD205 Antibody.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:100-200 | 1:10-50 |
Product Details
CD205 Antibody (YA9977) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to CD205 Antibody.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 205 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 198 kDa
Synthetic peptide corresponding to human CD205.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Immunohistochemical analysis of paraffin-embedded human follicular lymphoma tissue using CD205 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P810733, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded human melanoma tissue using CD205 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P810733, 1/150) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded human follicular lymphoma tissue using CD205 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P810733, 1/150) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded human thymus tissue using CD205 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P810733, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded human thymus tissue using CD205 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P810733, 1/150) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue using CD205 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P810733, 1/150) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Background
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Function
CD205 acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes
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Subcellular Localization
Membrane
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Expression
Tissue_Specificity: Expressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B-lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells. -
Isoforms & Post-Translational Modification
CD205 has 5 isoforms, O60449-1: amino acid length is 1722, molecular weight is 198311 Da (predicted); O60449-2: amino acid length is 1873, molecular weight is 215781 Da (predicted); Q8IX05-2: amino acid length is 174, molecular weight is 19562 Da (predicted); O60449-3: amino acid length is 1817, molecular weight is 209438 Da (predicted); Q8IX05-1: amino acid length is 232, molecular weight is 26183 Da (predicted).N-glycosylated
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SwissProt ID
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Synonyms
CD205, Cd205, SMU_610, spaP, Lymphocyte antigen 75, Ly-75, DEC-205
Documentation
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