AIF Antibody (YA835)(PBS only)

Cat. No.: HY-P80534A: Data Sheet User Guide for Antibodies
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AIF Antibody (YA835) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to AIF.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

AIF Antibody (YA835) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to AIF.

Host

Mouse

Molecular Weight

Predicted band size: 67 kDa;

Observed band size: 67 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O95831

Gene ID

9131 [NCBI]

Immunogen

Synthetic peptide corresponding to Human AIF.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Functions both as NADH oxidoreductase and as regulator of apoptosis (PubMed:17094969, PubMed:20362274, PubMed:23217327, PubMed:33168626). In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway (PubMed:20362274). Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains (By similarity). The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA (PubMed:20362274). Binds to DNA in a sequence-independent manner (PubMed:27178839). Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis (PubMed:17094969). Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells (PubMed:19418225). In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import (PubMed:26004228); Has NADH oxidoreductase activity. Does not induce nuclear apoptosis; Pro-apoptotic isoform

Subcellular Localization：Mitochondrion intermembrane space; Mitochondrion inner membrane; Cytoplasm; Nucleus; Cytoplasm, perinuclear region; Mitochondrion intermembrane space; Mitochondrion inner membrane; Mitochondrion; Cytoplasm, cytosol; Cytoplasm

Expression：
Tissue_specificity:Expression was observed in all tested tissues (PubMed: 16644725) . Detected in muscle and skin fibroblasts (protein level) (PubMed: 23217327) . Expressed in osteoblasts (protein level) (PubMed: 28842795) ; brain-specific; expressed in all tested tissues except brain tissue; isoform 5 is frequently downregulated in human cancers.

Induction:Strongly down-regulated in many tumor cells, up-regulated by gamma-irradiation

Isoforms & Post-Translational Modification：O95831 has 6 isomers: O95831-1: 66901 Da (predicted); O95831-2: 35638 Da (predicted); O95831-3: 66295 Da (predicted); O95831-4: 35384 Da (predicted); O95831-5: 28404 Da (predicted); O95831-6: 4812 Da (predicted).
Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner;Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation. Ubiquitination at Lys-255 by XIAP/BIRC4 blocks its ability to bind DNA and induce chromatin degradation, thereby inhibiting its ability to induce cell death

Subunit：Monomer (oxidized form). Homodimer (reduced form). Upon reduction with NADH, undergoes dimerization and forms tight, long-lived FADH2-NAD charge transfer complexes (CTC) resistant to oxidation (PubMed:20111043, PubMed:23217327, PubMed:24914854, PubMed:27818101). Also dimerizes with isoform 3 preventing its release from mitochondria (PubMed:20111043). Interacts with XIAP/BIRC4 (PubMed:17967870). Interacts (via N-terminus) with EIF3G (via C-terminus) (PubMed:17094969). Interacts with PRELID1 (PubMed:21364629). Interacts with CHCHD4; the interaction increases in presence of NADH (PubMed:26004228). Interacts with processed form of PARP1 (Poly [ADP-ribose] polymerase 1, processed C-terminus); interaction is mediated with poly-ADP-ribose chains attached to PARP1, promoting translocation into the nucleus (PubMed:33168626)

Research Field

Cell Biology

Synonyms

AIFM1; AIF; PDCD8; Apoptosis-inducing factor 1; mitochondrial; Programmed cell death protein 8

Documentation

User Guide for Antibodies (1077 KB)