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  5. Semaphorin 7A Antibody (YA3232)

Semaphorin 7A Antibody (YA3232)

Cat. No.: HY-P83487
COA
SDS
User Guide for Antibodies Technical Support

Semaphorin 7A Antibody (YA3232) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Semaphorin 7A.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Description

Description

Semaphorin 7A Antibody (YA3232) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Semaphorin 7A.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Masse moléculaire
Predicted band size: 75 kDa;
Observed band size: 75 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Recombinant protein of human Semaphorin 7a aa45-648.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
FC
FC: Flow Cytometry
1:200-1:500
IP
IP: Immunoprecipitation
1:20-1:50
Sensitivity Endogenous Pureté Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Livraison

Shipping with blue ice.
Background
Function：Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of pro-inflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes
Subcellular Localization：Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side
Expression：
Tissue_specificity:Protein levels were detected in keratinocytes and vascular endothelial cells of the skin. It was also expressed in fibroblasts, keratinocytes, melanocytes, placenta, testes, ovaries, spleen, brain, spinal cord, lungs, heart, adrenal glands, lymph nodes, thymus, intestines, and kidneys.

Induction:Up-regulated in UV-irradiated fibroblasts, but not in UV-irradiated keratinocytes
Isoforms & Post-Translational Modification：O75326 has 2 isomers: O75326-1: 74824 Da (predicted); O75326-2: 73364 Da (predicted).
Subunit：Interacts with ITGA1 and ITGB1 (Probable). Interacts with PLXNC1
Database

Entrez Gene: 8482 Human

SwissProt: O75326 Human

OMIM: 619874 Human

Research Field

-
Synonyms
JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1
Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Semaphorin 7A Antibody (YA3232) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Semaphorin 7A Antibody (YA3232)
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HY-P83487
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