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  4. Monoclonal Antibodies Recombinant Antibodies
  5. NRF-1 Antibody (YA260)

NRF-1 Antibody (YA260) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NRF-1.

For research use only. We do not sell to patients.

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50 μL In-stock
100 μL In-stock
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Top Publications Citing Use of Products

1 Publications Citing Use of MCE NRF-1 Antibody (YA260)

  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Documentation

Description

NRF-1 Antibody (YA260) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NRF-1.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 54 kDa;
Observed band size: 68 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Synthetic peptide corresponding to Human NRF1.The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Purity affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), 293T (lane 2, 20 μg), Jurkat (lane 3, 20 μg), Raw264.7 (lane 4, 20 μg), and 3T3 (lane 5, 20 μg) using Nrf1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background
Function：Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication
Subcellular Localization：Nucleus
Expression：
Tissue_specificity:It is widely expressed, with the strongest expression in skeletal muscle.
Isoforms & Post-Translational Modification：Q16656 has 4 isomers: Q16656-1: 53541 Da (predicted); Q16656-2: 46157 Da (predicted); Q16656-3: 36051 Da (predicted); Q16656-4: 55674 Da (predicted).
Phosphorylation enhances DNA binding
Subunit：Homodimer. Binds DNA as a dimer. Interacts with PPRC1
RRID
Database

Entrez Gene: 4899 Human ; 18181 Mouse ;

SwissProt: Q16656 Human ; Q9WU00 Mouse ; Q62792 Rat

OMIM: 600879 Human

Research Field

Epigenetics and Nuclear Signaling
Synonyms
alpha pal; alpha palindromic binding protein; Alpha palindromic-binding protein; Alpha-pal; locus control region factor 1; NFE2 related factor 1; NRF-1; Nrf1; NRF1_HUMAN; Nuclear respiratory factor 1; transcription factor 11.
Documentation
SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

NRF-1 Antibody (YA260) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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NRF-1 Antibody (YA260)
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HY-P80769
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