IRF8 Antibody (YA3121)
(Synonyms: ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP)
Based on 1 Customer Validation
IRF8 Antibody (YA3121) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRF8.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IHC-P, IP
-
Reactivity :
Human
-
Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
IP
IP: Immunoprecipitation
|
|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:20 |
Product Details
IRF8 Antibody (YA3121) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRF8.
-
Host Rabbit
-
Clonality Recombinant,Monoclonal
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 48 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 48 kDa
Recombinant protein of human IRF8
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
IRF8 transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Can both act as a transcriptional activator or repressor. Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes. Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection. Positively regulates macroautophagy in dendritic cells. Acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1[1][2].
-
Subcellular Localization
Nucleus; Cytoplasm
-
Expression
Tissue_specificity:Predominantly expressed in lymphoid tissues
Induction:By IFNG/IFN-gamma. Negatively regulated by microRNA-155 (miR155) -
Subunit
Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct (By similarity). Interacts with COPS2. Interacts with SPI1 (PubMed:33951726)
-
SwissProt ID
-
Synonyms
ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP
-
Research Field
Epigenetics and Nuclear Signaling
Documentation
Powered by Bioz
