Monocarboxylic Acid Transporter 1 Antibody (YA3244)

製品番号: HY-P83499: データシート SDS COA User Guide for Antibodies
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Monocarboxylic Acid Transporter 1 Antibody (YA3244) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Monocarboxylic Acid Transporter 1.

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容量	価格（税別）	在庫状況	数量
20 μL	$126	在庫あり	Estimated Time of Arrival: December 31
50 μL	$185	在庫あり	Estimated Time of Arrival: December 31
100 μL	$300	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

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おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Monocarboxylic Acid Transporter 1 Antibody (YA3244) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Monocarboxylic Acid Transporter 1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 54 kDa;

Observed band size: 54 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P53985

Gene ID

6566 [NCBI]

Immunogen

A synthetic peptide of human Monocarboxylic acid transporter 1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), K562 (lane 4, 15 μg), Raji (lane 5, 15 μg), and 293 (lane 6, 15 μg) using Monocarboxylic Acid Transporter 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using Monocarboxylic Acid Transporter 1 Antibody (YA3244) (HY-P83499) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Bidirectional proton-coupled monocarboxylate transporter (PubMed:12946269, PubMed:32946811, PubMed:33333023). Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, acetate and the ketone bodies acetoacetate and beta-hydroxybutyrate, and thus contributes to the maintenance of intracellular pH (PubMed:12946269, PubMed:33333023). The transport direction is determined by the proton motive force and the concentration gradient of the substrate monocarboxylate. MCT1 is a major lactate exporter (By similarity). Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis (By similarity). Facilitates the protonated monocarboxylate form of succinate export, that its transient protonation upon muscle cell acidification in exercising muscle and ischemic heart (PubMed:32946811). Functions via alternate outward- and inward-open conformation states. Protonation and deprotonation of 309-Asp is essential for the conformational transition (PubMed:33333023)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein; Apical cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Widely expressed (PubMed:12115955, PubMed:15505343, PubMed:15901598) . Detected in heart and in blood lymphocytes and monocytes (at protein level) (PubMed:15505343)

Isoforms & Post-Translational Modification：P53985 has 2 isomers: P53985-1: 53944 Da (predicted); P53985-2: 46234 Da (predicted).

Subunit：Interacts with EMB; interaction mediates SLC16A1 targeting to the plasma membrane (By similarity). Interacts with isoform 2 of BSG; interaction mediates SLC16A1 targeting to the plasma membrane (PubMed:17127621, PubMed:25957687, PubMed:33333023)

RRID

AB_3105609

Database

Entrez Gene: 6566 Human

SwissProt: P53985 Human

OMIM: 245340 Human

Research Field

Cancer

Synonyms

HHF7; MCT 1; MCT; Slc16a1

ドキュメンテーション

Select Batch:

データシート (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)