GBP1 Antibody (YA2930)(PBS only)

Cat. No.: HY-P83185A: Data Sheet User Guide for Antibodies
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GBP1 Antibody (YA2930) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GBP1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

GBP1 Antibody (YA2930) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GBP1.

Host

Rabbit

Molecular Weight

Predicted band size: 68 kDa;

Observed band size: 68 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P32455

Gene ID

2633 [NCBI]

Immunogen

A synthetic peptide of human GBP1 aa560-573.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens (PubMed:16511497, PubMed:22106366, PubMed:29144452, PubMed:31268602, PubMed:32510692, PubMed:32581219, PubMed:37797010, PubMed:7512561). Hydrolyzes GTP to GMP in two consecutive cleavage reactions: GTP is first hydrolyzed to GDP and then to GMP in a processive manner (PubMed:16511497, PubMed:32510692, PubMed:7512561). Following infection, recruited to the pathogen-containing vacuoles or vacuole-escaped bacteria and promotes both inflammasome assembly and autophagy (PubMed:29144452, PubMed:31268602). Acts as a positive regulator of inflammasome assembly by facilitating the detection of inflammasome ligands from pathogens (PubMed:31268602, PubMed:32510692, PubMed:32581219). Involved in the lysis of pathogen-containing vacuoles, releasing pathogens into the cytosol (By similarity). Following pathogen release in the cytosol, forms a protein coat in a GTPase-dependent manner that encapsulates pathogens and promotes the detection of ligands by pattern recognition receptors (PubMed:32510692, PubMed:32581219). Plays a key role in inflammasome assembly in response to infection by Gram-negative bacteria: following pathogen release in the cytosol, forms a protein coat that encapsulates Gram-negative bacteria and directly binds to lipopolysaccharide (LPS), disrupting the O-antigen barrier and unmasking lipid A that is that detected by the non-canonical inflammasome effector CASP4/CASP11 (PubMed:32510692, PubMed:32581219). Also promotes recruitment of proteins that mediate bacterial cytolysis, leading to release double-stranded DNA (dsDNA) that activates the AIM2 inflammasome (PubMed:31268602). Involved in autophagy by regulating bacteriolytic peptide generation via its interaction with ubiquitin-binding protein SQSTM1, which delivers monoubiquitinated proteins to autolysosomes for the generation of bacteriolytic peptides (By similarity). Confers protection to several pathogens, including the bacterial pathogens L.monocytogenes and M.bovis BCG as well as the protozoan pathogen T.gondii (PubMed:31268602). Exhibits antiviral activity against influenza virus (PubMed:22106366)

Subcellular Localization：Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side; Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side; Cell membrane; Lipid-anchor; Cytoplasmic side; Cytoplasm, cytosol; Secreted

Expression：
Induction:By IFNG during macrophage activation, and by TNF and IL1B

Subunit：Homodimer; homodimerization occurs upon GTP-binding and is required for the second hydrolysis step from GDP to GMP (PubMed:10970849, PubMed:16511497). Undergoes conformational changes and oligomerization upon GTP-binding and hydrolysis (PubMed:28645896, PubMed:32510692, PubMed:32581219). Heterodimer with other family members, including GBP2, GBP3, GBP4 and GBP5 (PubMed:10970849, PubMed:16511497, PubMed:32581219). Dimerization regulates subcellular location to membranous structures (PubMed:21151871). Interacts with SQSTM1 (By similarity). Interacts (when phosphorylated) with 14-3-3 protein sigma (SFN); leading to GBP1 retention in the cytosol and inactivation (PubMed:37797010)

Research Field

Immunology

Synonyms

GTP-binding protein 1

Documentation

User Guide for Antibodies (1077 KB)