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Wnt5b Antibody (YA653)(PBS only)

(Synonyms: Wnt5b)

Wnt5b Antibody (YA653) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Wnt5b.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Wnt5b Antibody (YA653)(PBS only)
  • Wnt5b Antibody (YA653)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Wnt5b Antibody (YA653) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Wnt5b.

  • Host Mouse
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 40 kDa
Immunogen

Synthetic peptide corresponding to Human Wnt5b.The exact sequence is proprietary to MCE.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Wnt5b Antibody (YA653)(PBS only)
    Western blot analysis of extracts from Hela(lane 2(20ug) , HepG2(lane 3(20ug) and HEK293(lane 4(20ug) using Wnt5b Antibody (HY-P80934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Wnt5b is a Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Subunit

    Interacts with PORCN

  • SwissProt ID

    Q9H1J7

  • Gene ID
    81029 [NCBI]
  • Synonyms

    Wnt5b

  • Research Field

    Neuroscience

Wnt5b Antibody (YA653)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , HepG2(lane 3(20ug) and HEK293(lane 4(20ug) using Wnt5b Antibody (HY-P80934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Wnt5b Antibody (YA653)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Wnt5b Antibody (YA653)(PBS only)
  • Wnt5b Antibody (YA653)(PBS only) Product Image
  • Experimental Validation Results for Wnt5b Antibody (YA653)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , HepG2(lane 3(20ug) and HEK293(lane 4(20ug) using Wnt5b Antibody (HY-P80934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Wnt5b Antibody (YA653)(PBS only)
  • Wnt5b Antibody (YA653)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) , HepG2(lane 3(20ug) and HEK293(lane 4(20ug) using Wnt5b Antibody (HY-P80934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Wnt5b Antibody (YA653)(PBS only) Product Image WB