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Phospho-LRRK2(Ser935) Antibody (YA3424)

(Synonyms: AURA17; Dardarin antibody; ; Leucine rich repeat kinase 2; LRRK 2 antibody; LRRK2; LRRK2_HUMAN; PARK 8; PARK8; RIPK7; ROCO 2; ROCO2)
Customer Review

Based on 1 Customer Validation

Phospho-LRRK2(Ser935) Antibody (YA3424) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-LRRK2(Ser935).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-LRRK2(Ser935) Antibody (YA3424)
  • Experimental Validation Results for Phospho-LRRK2(Ser935) Antibody (YA3424)
  • Phospho-LRRK2(Ser935) Antibody (YA3424) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Phospho-LRRK2(Ser935) Antibody (YA3424) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-LRRK2(Ser935).

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 286 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 286 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Phospho-LRRK2 (S935).

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3676601

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-LRRK2(Ser935) Antibody (YA3424)
    Immunocytochemistry analysis of A549 cells labeling Phospho-LRRK2(Ser935) with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-LRRK2(Ser935) Antibody (YA3424)
    Immunocytochemistry analysis of A549 cells labeling Phospho-LRRK2(Ser935) with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/100 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Leucine-rich repeat kinase 2 (LRRK2) is a ubiquitously expressed member of the ROCO protein family. LRRK2 is a complex, multidomain protein containing kinase and GTPase enzymatic activities and multiple protein-protein interaction domains. LRRK2 is the genetic cause of both familial and idiopathic Parkinson's disease (PD), and it is associated with neuronal death, vesicle trafficking, mitochondrial dysfunction, and inflammation. LRRK2 is a very large protein comprised of 2527 amino acids which has been determined to contain multiple functional domains, including armadillo (ARM), ankyrin-repeats (ANK), leucine-rich repeats (LRR), Ras of complex proteins (ROC), C-terminal of Roc (COR), MAPK-like kinase, and WD40 motifs. Mutations in LRRK2 represent a significant component of both sporadic and familial PD. Pathogenic mutations cluster in the enzymatic domains of LRRK2, and kinase activity seems to correlate with cytotoxicity, suggesting the possibility of kinase-based therapeutic strategies for LRRK2-associated PD. The best-characterized mutation to date, G2019S, leads to increased kinase activity, and mutations in the GTPase domain, such as R1441C and R1441G, have also been reported to influence kinase activity.

  • Subcellular Localization

    Cytoplasmic vesicle; Perikaryon; Golgi apparatus membrane; Peripheral membrane protein; Cell projection, axon; Cell projection, dendrite; Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Endosome; Lysosome; Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasm, cytoskeleton; Cytoplasmic vesicle, phagosome

  • Expression


    Tissue_specificity:It is expressed (protein level) in pyramidal neurons of all cortical layers of the visual cortex, neurons of the substantia nigra pars compacta, and neurons of the putamen of the caudate nucleus. It is also expressed (protein level) in neutrophils (PubMed:29127255) . It is also expressed in the brain. It is expressed throughout the adult brain, but at lower levels than in the heart and liver. It is also expressed in the placenta, lungs, skeletal muscle, kidneys, and pancreas. In the brain, it is expressed in the cerebellum, cerebral cortex, medulla oblongata, occipital pole of the spinal cord, frontal lobe, temporal lobe, and putamen. Expression is particularly strong in dopaminergic regions of the brain.

  • Subunit

    Homodimer (PubMed:18230735, PubMed:22952686, PubMed:30635421, PubMed:38127736). Homotetramer; when activated by GTP-bound RAB29 (PubMed:38127736). Interacts with PRKN, PRDX3, and TPCN2 (PubMed:16352719, PubMed:21850687, PubMed:22012985). Interacts with VPS35 (PubMed:23395371). Interacts (via N-terminus) with RAB29; this interaction is direct and stimulates kinase activity (PubMed:23395371, PubMed:38127736). Interacts (via ROC domain) with SEC16A (PubMed:25201882). Interacts with APP; interaction promotes phosphorylation of 'Thr-743' of APP (PubMed:28720718). Interacts with MAPT (PubMed:26014385). Interacts with RAB8A, RAB10, and RAB12 (PubMed:26824392). Interacts (via N-terminus) with RAB32 (PubMed:38127736). Interacts with YWHAG; this interaction is dependent on phosphorylation of Ser-910 and either Ser-935 or Ser-1444 (PubMed:28202711). Interacts with SFN; this interaction is dependent on phosphorylation of Ser-910 and/or Ser-935 (PubMed:28202711)

  • SwissProt ID

    Q5S007

  • Gene ID
    120892 [NCBI]
  • Synonyms

    AURA17; Dardarin antibody; ; Leucine rich repeat kinase 2; LRRK 2 antibody; LRRK2; LRRK2_HUMAN; PARK 8; PARK8; RIPK7; ROCO 2; ROCO2

  • Research Field

    Neuroscience

Phospho-LRRK2(Ser935) Antibody (YA3424) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Phospho-LRRK2(Ser935) with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Phospho-LRRK2(Ser935) with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/100 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-LRRK2(Ser935) Antibody (YA3424) Product Image
    1/16
  • Experimental Validation Results for Phospho-LRRK2(Ser935) Antibody (YA3424)
  • Experimental Validation Results for Phospho-LRRK2(Ser935) Antibody (YA3424)
  • Phospho-LRRK2(Ser935) Antibody (YA3424) Product Image
  • Experimental Validation Results for Phospho-LRRK2(Ser935) Antibody (YA3424)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Phospho-LRRK2(Ser935) with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-LRRK2(Ser935) Antibody (YA3424)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Phospho-LRRK2(Ser935) with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/100 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-LRRK2(Ser935) Antibody (YA3424)
  • Experimental Validation Results for Phospho-LRRK2(Ser935) Antibody (YA3424)
  • Phospho-LRRK2(Ser935) Antibody (YA3424) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling Phospho-LRRK2(Ser935) with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-LRRK2(Ser935) Antibody (YA3424) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling Phospho-LRRK2(Ser935) with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-LRRK2(Ser935) Antibody (HY-P83076A) at 1/100 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-LRRK2(Ser935) Antibody (YA3424) Product Image ICC
  • Phospho-LRRK2(Ser935) Antibody (YA3424) Product Image ICC