TAP2 Antibody
(Synonyms: ABCB3, PSF2, RING11, Y1, TAP2, Antigen peptide transporter 2, APT2, ATP-binding cassette sub-family B member 3, Peptide supply factor 2, Peptide transporter PSF2, Peptide transporter TAP2, Peptide transporter involved in antigen processing 2, Really interesting new gene 11 protein, PSF-2)TAP2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to TAP2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
TAP2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to TAP2.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 75 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 72; 75 kDa
Purified recombinant protein of human TAP2
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Soulution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TAP2 is an ABC transporter associated with antigen processing. In complex with TAP1 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle. Typically transports intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome. Binds peptides with free N- and C-termini, the first three and the C-terminal residues being critical. Preferentially selects peptides having a highly hydrophobic residue at position 3 and hydrophobic or charged residues at the C-terminal anchor. Proline at position 2 has the most destabilizing effect. As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation[1][2][3][4][5][6][7].
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Subcellular Localization
Endoplasmic reticulum membrane
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Subunit
Heterodimer of TAP1 and TAP2 (TAP1-TAP2).
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SwissProt ID
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Synonyms
ABCB3, PSF2, RING11, Y1, TAP2, Antigen peptide transporter 2, APT2, ATP-binding cassette sub-family B member 3, Peptide supply factor 2, Peptide transporter PSF2, Peptide transporter TAP2, Peptide transporter involved in antigen processing 2, Really interesting new gene 11 protein, PSF-2
Documentation
[1]. Grossmann N, et al. Mechanistic determinants of the directionality and energetics of active export by a heterodimeric ABC transporter. Nat Commun. 2014 Nov 7;5:5419. [Content Brief]
[2]. Fischbach H, et al. Ultrasensitive quantification of TAP-dependent antigen compartmentalization in scarce primary immune cell subsets. Nat Commun. 2015 Feb 6;6:6199. [Content Brief]
[3]. Gorbulev S, et al. Allosteric crosstalk between peptide-binding, transport, and ATP hydrolysis of the ABC transporter TAP. Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3732-7. [Content Brief]
[4]. van Endert PM, et al. The peptide-binding motif for the human transporter associated with antigen processing. J Exp Med. 1995 Dec 1;182(6):1883-95. [Content Brief]
[5]. Uebel S, et al. Recognition principle of the TAP transporter disclosed by combinatorial peptide libraries. Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):8976-81. [Content Brief]
[6]. Kelly A, et al. Assembly and function of the two ABC transporter proteins encoded in the human major histocompatibility complex. Nature. 1992 Feb 13;355(6361):641-4. [Content Brief]
[7]. Blees A, et al. Assembly of the MHC I peptide-loading complex determined by a conserved ionic lock-switch. Sci Rep. 2015 Nov 27;5:17341. [Content Brief]
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