2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies Flow Cytometry Antibodies
  5. E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA020)

E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA020)

Cat. No.: HY-P80365
User Guide for Antibodies Technical Support

E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA020) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to E1 Ubiquitin Activating Enzyme 1/UBA1.

For research use only. We do not sell to patients.

Size Price Stock
20 μL Ask For Quote & Lead Time
50 μL Ask For Quote & Lead Time
100 μL Ask For Quote & Lead Time

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA020) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to E1 Ubiquitin Activating Enzyme 1/UBA1.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 118 kDa;
Observed band size: 118 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse
SwissProt ID
Gene ID
Immunogen

Synthetic peptide corresponding to Human UBA1.AA range:1009-1058.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:2000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-1:500
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Purity Protein A affinity purified.
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1447181, PubMed:1606621, PubMed:33108101). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334)
Subcellular Localization：Cytoplasm; Mitochondrion; Nucleus; Nucleus; Cytoplasm
Expression：
Tissue_specificity:Detected in red blood cells (protein level) . Widely present.
Subunit：Monomer (By similarity). Interacts with GAN (via BTB domain)
Database

Entrez Gene: 7317 Human ; 22201 Mouse ; 314432 Rat

SwissProt: P22314 Human ; Q02053 Mouse ; Q5U300 Rat

OMIM: 301830 Human

Research Field

Cell Biology
Documentation
User Guide for Antibodies (1077 KB)

E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA020) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA020)
Cat. No.:
HY-P80365
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.