Tight Junction Protein ZO 3 Antibody (YA2456)(PBS only)

Cat. No.: HY-P82711A: Data Sheet User Guide for Antibodies
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Tight Junction Protein ZO 3 Antibody (YA2456) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tight Junction Protein ZO 3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Tight Junction Protein ZO 3 Antibody (YA2456) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tight Junction Protein ZO 3.

Host

Rabbit

Molecular Weight

Predicted band size: 101 kDa;

Observed band size: 140 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

O95049

Gene ID

27134 [NCBI]

Immunogen

A synthetic peptide of human Zonula occludens protein 3

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:16129888). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions (PubMed:16129888). Promotes cell-cycle progression through the sequestration of cyclin D1 (CCND1) at tight junctions during mitosis which prevents CCND1 degradation during M-phase and enables S-phase transition (PubMed:21411630). With TJP1 and TJP2, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). Contrary to TJP2, TJP3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment (By similarity)

Subcellular Localization：Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell junction, tight junction; Nucleus

Expression：
Induction:Exhibits enhanced expression in matured epithelial layers (PubMed:23608536) . Apical leptin, Staphylococcus aureus alpha-toxin and Pseudomonas aeruginosa acyl-homoserine lactone 3O-C12-HSl lower expression levels, altering junctional integrity in intestinal cells (PubMed:20434232, PubMed:22354024, PubMed:24314862)

Isoforms & Post-Translational Modification：O95049 has 4 isomers: O95049-1: 101397 Da (predicted); O95049-3: 103424 Da (predicted); O95049-4: 102415 Da (predicted); O95049-5: 97488 Da (predicted).
Phosphorylated (By similarity)

Subunit：Interacts with UBN1 (PubMed:20434232). Interacts with FASLG (PubMed:19807924). Interacts with CCND1 (PubMed:21411630)

Research Field

Signal Transduction

Synonyms

ZO3; ZO-3

Documentation

User Guide for Antibodies (1077 KB)