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ZAP70 Antibody (YA651)

(Synonyms: ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase)
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Based on 1 Customer Validation

ZAP70 Antibody (YA651) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to ZAP70.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ZAP70 Antibody (YA651)
  • ZAP70 Antibody (YA651) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

ZAP70 Antibody (YA651) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to ZAP70.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 70 kDa
Species Reactivity Database

Entrez Gene: 7535 Human

SwissProt: P43403 Human

Immunogen

Synthetic peptide corresponding to Human ZAP-70.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ZAP70 Antibody (YA651)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), MOLT-4 (lane 2, 15 μg), and CCRF-CEM (lane 3, 15 μg) using ZAP70 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    ZAP70 (zeta-chain-associated protein kinase 70) is a cytoplasmic protein tyrosine kinase that functions as a central signaling mediator downstream of the T-cell receptor (TCR) and is required for the initiation of antigen receptor-driven T-cell responses[1]. Mechanistically, TCR engagement leads to phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs)， recruitment of ZAP70 through its tandem SH2 domains, and subsequent phosphorylation of adaptor proteins including LAT and SLP-76, thereby propagating signaling pathways that regulate T-cell activation, differentiation, proliferation, and cytokine production[1][5]. Because of this pivotal role in adaptive immunity, ZAP70 is essential for normal thymocyte development as well as peripheral T-cell function[1][5]. In disease settings, loss-of-function mutations in ZAP70 cause a combined immunodeficiency characterized by profound defects in T-cell signaling, markedly reduced CD8+ T-cell populations, recurrent infections, and immune dysregulation[2][3]. Altered ZAP70 signaling has also been linked to autoimmune phenotypes, highlighting the requirement for tightly regulated TCR signaling thresholds during immune homeostasis[2]. Compared with the closely related kinase SYK, ZAP70 exhibits a specialized role in T-cell receptor signaling, whereas SYK displays broader expression and higher intrinsic kinase activity in multiple immune cell types[6]. For experimental research, ZAP70 serves as a widely used molecular node for dissecting TCR signal transduction networks and immune-cell activation mechanisms, and its kinase activity can be selectively inhibited by compounds such as epigallocatechin gallate (EGCG) in cellular studies[4].

  • Subcellular Localization

    Cytoplasm; Cell membrane; Peripheral membrane protein

  • Expression


    Tissue_specificity:It is expressed in T cells and natural killer cells. It is also present in early thymocytes and pre-B cells/pre-B cells.

  • Isoforms & Post-Translational Modification

    P43403 has 3 isomers: P43403-1: 69872 Da (predicted); P43403-2: 35647 Da (predicted); P43403-3: 55873 Da (predicted).
    Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive function on T-lymphocyte activation whereas Tyr-292 has a negative regulatory role. Within the C-terminal kinase domain, Tyr-492 and Tyr-493 are phosphorylated after TCR induction, Tyr-492 playing a negative regulatory role and Tyr-493 a positive. Tyr-493 is dephosphorylated by PTN22;Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B

  • Subunit

    Interacts with CD247/CD3Z; this interaction docks ZAP70 at the stimulated TCR (PubMed:1423621, PubMed:26783323, PubMed:7659156). Interacts with NFAM1 (PubMed:15143214).

  • SwissProt ID

    P43403

  • Gene ID
    7535 [NCBI]
  • Synonyms

    ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

  • Research Field

    Immunology

[1]. Noraz N, et al. Alternative antigen receptor (TCR) signaling in T cells derived from ZAP-70-deficient patients expressing high levels of Syk. J Biol Chem. 2000;275(21):15832-15838.

[2]. Fernández-Aguilar LM, et al. A Story of Kinases and Adaptors: The Role of Lck, ZAP-70 and LAT in Switch Panel Governing T-Cell Development and Activation. Biology (Basel). 2023;12(9):1163.

[3]. Ashouri JF, et al. ZAP70, too little, too much can lead to autoimmunity. Immunol Rev. 2022 May;307(1):145-160. [Content Brief]

[4]. Sharifinejad N, et al. Clinical, Immunological, and Genetic Features in 49 Patients With ZAP-70 Deficiency: A Systematic Review. Front Immunol. 2020 May 5;11:831. [Content Brief]

[5]. Wang H, et al. ZAP-70: an essential kinase in T-cell signaling. Cold Spring Harb Perspect Biol. 2010;2(5):a002279. [Content Brief]

[6]. ZAP70 gene information from NCBI.

[7]. Wang H, et al. ZAP-70: an essential kinase in T-cell signaling. Cold Spring Harb Perspect Biol. 2010 May;2(5):a002279. [Content Brief]

[8]. Zhang W, et al. LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation. Cell. 1998 Jan 9;92(1):83-92. [Content Brief]

[9]. Arpaia E, et al. Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase. Cell. 1994 Mar 11;76(5):947-58. [Content Brief]

[10]. Wiestner A, et al. ZAP-70 expression identifies a chronic lymphocytic leukemia subtype with unmutated immunoglobulin genes, inferior clinical outcome, and distinct gene expression profile. Blood. 2003 Jun 15;101(12):4944-51. [Content Brief]

[11]. Noraz N, et al. Alternative antigen receptor (TCR) signaling in T cells derived from ZAP-70-deficient patients expressing high levels of Syk. J Biol Chem. 2000 May 26;275(21):15832-8. [Content Brief]

[12]. Visperas PR, et al. Identification of Inhibitors of the Association of ZAP-70 with the T Cell Receptor by High-Throughput Screen. SLAS Discov. 2017 Mar;22(3):324-331. [Content Brief]

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ZAP70 Antibody (YA651) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), MOLT-4 (lane 2, 15 μg), and CCRF-CEM (lane 3, 15 μg) using ZAP70 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ZAP70 Antibody (YA651) Product Image
    1/16
  • Experimental Validation Results for ZAP70 Antibody (YA651)
  • ZAP70 Antibody (YA651) Product Image
  • Experimental Validation Results for ZAP70 Antibody (YA651)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), MOLT-4 (lane 2, 15 μg), and CCRF-CEM (lane 3, 15 μg) using ZAP70 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ZAP70 Antibody (YA651)
  • ZAP70 Antibody (YA651) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), MOLT-4 (lane 2, 15 μg), and CCRF-CEM (lane 3, 15 μg) using ZAP70 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ZAP70 Antibody (YA651) Product Image WB