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Cytokeratin 5 Antibody (YA3509)

(Synonyms: CK-5; CK5; Cytokeratin-5; Cytokeratin5; DDD; DDD1; EBS2; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K2C5_HUMAN; K5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); Keratin 5; Keratin ; keratin complex 2, basic, gene 5; keratin, type II cytoskeletal 5 ; Keratin-5; Keratin5; KRT 5; Krt5; KRT5A; type II cytoskeletal 5 ; Type-II keratin Kb5; )
Customer Review

Based on 1 Customer Validation

Cytokeratin 5 Antibody (YA3509) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC FC
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
  • Cytokeratin 5 Antibody (YA3509) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:5000 1:50-1:1500 1:200 1:500 1:1000

Product Details

Description

Cytokeratin 5 Antibody (YA3509) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 5.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 57 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within human Cytokeratin 5 aa 70-110.

Sensitivity

Endogenous

Purification

Affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103013

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
    Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using Cytokeratin 5 (HY-P80984) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
    Immunocytochemistry analysis of Hela cells labeling Cytokeratin 5 with Cytokeratin 5 Antibody (HY-P80984)at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
    Immunocytochemistry analysis of A431 cells labeling Cytokeratin 5 with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
    Flow cytometric analysis of 1X10^6 A431 cells labeling Cytokeratin 5 Antibody (HY-P80984, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Cytokeratin 5 is required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress. Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Expression (protein level) in corneal epithelial cells (PubMed: 26758872) . Expression (protein level) in keratinocytes (PubMed: 20128788, PubMed: 31302245) .

  • Subunit

    Heterodimer of a type I and a type II keratin (PubMed:22705788, PubMed:31995743). Heterodimer with type I keratin KRT25 leading to the formation of keratin intermediate filament (KIF) network (PubMed:28899683). Forms a heterodimer (via 2B domains) with KRT14 (via 2B domains) (PubMed:22705788, PubMed:24940650, PubMed:31995743). Interacts with PLEC isoform 1C, when in a heterodimer with KRT14 (PubMed:24940650). Interacts with TCHP (PubMed:15731013). Interacts with EPPK1 (By similarity). Interacts with AMELX (By similarity). Interacts with PKP1 (via N-terminus) and PKP2 (PubMed:10852826)

  • SwissProt ID

    P13647

  • Gene ID
    3852 [NCBI]
  • Synonyms

    CK-5; CK5; Cytokeratin-5; Cytokeratin5; DDD; DDD1; EBS2; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K2C5_HUMAN; K5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); Keratin 5; Keratin ; keratin complex 2, basic, gene 5; keratin, type II cytoskeletal 5 ; Keratin-5; Keratin5; KRT 5; Krt5; KRT5A; type II cytoskeletal 5 ; Type-II keratin Kb5;

Cytokeratin 5 Antibody (YA3509) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using Cytokeratin 5 (HY-P80984) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Cytokeratin 5 with Cytokeratin 5 Antibody (HY-P80984)at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Cytokeratin 5 with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 A431 cells labeling Cytokeratin 5 Antibody (HY-P80984, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Cytokeratin 5 Antibody (YA3509) Product Image
    1/16
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
  • Cytokeratin 5 Antibody (YA3509) Product Image
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using Cytokeratin 5 (HY-P80984) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Cytokeratin 5 with Cytokeratin 5 Antibody (HY-P80984)at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Cytokeratin 5 with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 A431 cells labeling Cytokeratin 5 Antibody (HY-P80984, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
  • Experimental Validation Results for Cytokeratin 5 Antibody (YA3509)
  • Cytokeratin 5 Antibody (YA3509) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using Cytokeratin 5 (HY-P80984) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Cytokeratin 5 Antibody (YA3509) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Cytokeratin 5 with Cytokeratin 5 Antibody (HY-P80984)at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cytokeratin 5 Antibody (YA3509) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling Cytokeratin 5 with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 5 Antibody (HY-P80984) at 1/500 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cytokeratin 5 Antibody (YA3509) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 A431 cells labeling Cytokeratin 5 Antibody (HY-P80984, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Cytokeratin 5 Antibody (YA3509) Product Image WB
  • Cytokeratin 5 Antibody (YA3509) Product Image ICC
  • Cytokeratin 5 Antibody (YA3509) Product Image ICC
  • Cytokeratin 5 Antibody (YA3509) Product Image FC