Copy Link Print Information

Cytochrome P450 2E1 Antibody (YA2302)

(Synonyms: CYP2E1; CYP2E; Cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; Cytochrome P450-J)
Customer Review

Based on 1 Customer Validation

Cytochrome P450 2E1 Antibody (YA2302) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytochrome P450 2E1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cytochrome P450 2E1 Antibody (YA2302)
  • Experimental Validation Results for Cytochrome P450 2E1 Antibody (YA2302)
  • Cytochrome P450 2E1 Antibody (YA2302) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Cytochrome P450 2E1 Antibody (YA2302) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytochrome P450 2E1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 57 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human CYP2E1 aa52-114.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104658

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cytochrome P450 2E1 Antibody (YA2302)
    Western blot analysis was performed on extracts from Rat liver (lane 1, 15 μg), and MCF-7 (lane 2, 15 μg) using Cytochrome P450 2E1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Cytochrome P450 2E1 Antibody (YA2302)
    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Cytochrome P450 2E1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Cytochrome P450 2E1 is an A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics (Probable)[1][2].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Peripheral membrane protein; Microsome membrane; Peripheral membrane protein; Mitochondrion inner membrane; Peripheral membrane protein

  • Expression


    Induction:By ethanol and isoniazid

  • Subunit

    Interacts with chaperones HSP70 and HSP90; this interaction is required for initial targeting to mitochondria

  • SwissProt ID

    P05181

  • Gene ID
    1571 [NCBI]
  • Synonyms

    CYP2E1; CYP2E; Cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; Cytochrome P450-J

  • Research Field

    Cardiovascular

Cytochrome P450 2E1 Antibody (YA2302) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Rat liver (lane 1, 15 μg), and MCF-7 (lane 2, 15 μg) using Cytochrome P450 2E1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Cytochrome P450 2E1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Cytochrome P450 2E1 Antibody (YA2302) Product Image
    1/16
  • Experimental Validation Results for Cytochrome P450 2E1 Antibody (YA2302)
  • Experimental Validation Results for Cytochrome P450 2E1 Antibody (YA2302)
  • Cytochrome P450 2E1 Antibody (YA2302) Product Image
  • Experimental Validation Results for Cytochrome P450 2E1 Antibody (YA2302)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Rat liver (lane 1, 15 μg), and MCF-7 (lane 2, 15 μg) using Cytochrome P450 2E1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Cytochrome P450 2E1 Antibody (YA2302)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Cytochrome P450 2E1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cytochrome P450 2E1 Antibody (YA2302)
  • Experimental Validation Results for Cytochrome P450 2E1 Antibody (YA2302)
  • Cytochrome P450 2E1 Antibody (YA2302) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Rat liver (lane 1, 15 μg), and MCF-7 (lane 2, 15 μg) using Cytochrome P450 2E1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Cytochrome P450 2E1 Antibody (YA2302) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Cytochrome P450 2E1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Cytochrome P450 2E1 Antibody (YA2302) Product Image WB
  • Cytochrome P450 2E1 Antibody (YA2302) Product Image WB