ALKBH2 Antibody (YA7512)
ALKBH2 Antibody (YA7512) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to ALKBH2.
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Host:
Rabbit
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Isotype:
IgG,Kappa
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Application:
WB, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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|---|---|---|
| Dilution Ratio | 1:1000-1:5000 | 1:100-1:500 |
Product Details
ALKBH2 Antibody (YA7512) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to ALKBH2.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 29 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 29 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG,Kappa
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ALKBH2 is a Dioxygenase that repairs alkylated nucleic acid bases by direct reversal oxidative dealkylation. Can process both double-stranded (ds) and single-stranded (ss) DNA substrates, with a strong preference for dsDNA. Uses molecular oxygen, 2-oxoglutarate and iron as cofactors to oxidize the alkyl groups that are subsequently released as aldehydes, regenerating the undamaged bases. Probes the base pair stability, locates a weakened base pair and flips the damaged base to accommodate the lesion in its active site for efficient catalysis. Repairs monoalkylated bases, specifically N1-methyladenine and N3-methylcytosine, as well as higher order alkyl adducts such as bases modified with exocyclic bridged adducts known as etheno adducts including 1,N6-ethenoadenine, 3,N4-ethenocytosine and 1,N2-ethenoguanine. Acts as a gatekeeper of genomic integrity under alkylation stress. Efficiently repairs alkylated lesions in ribosomal DNA (rDNA). These lesions can cause ss- and dsDNA strand breaks that severely impair rDNA transcription. In a response mechanism to DNA damage, associates with PCNA at replication forks to repair alkylated adducts prior to replication[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Nucleus,Nucleus, nucleolus,Nucleus, nucleoplasm
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Expression
Tissue_Specificity: Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder -
Isoforms & Post-Translational Modification
Q6NS38 has two isomers: Q6NS38-1: 29322 Da (predicted); Q6NS38-2: 17083 Da (predicted).
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Subunit
Interacts with PCNA homotrimer; this interaction is enhanced during the S-phase of the cell cycle (PubMed:19736315, PubMed:23972994, PubMed:26408825)
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SwissProt ID
Documentation
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